LOCUS       sequence-85904-e        1862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1106..1115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1112..1822
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 accggactca gatctcgagg ccaccatggc tgagccccgc caggagttcg aagtgatgga
       61 agatcacgct gggacgtacg ggttggggga caggaaagat caggggggct acaccatgca
      121 ccaagaccaa gagggtgaca cggacgctgg cctgaaagct gaagaagcag gcattggaga
      181 cacccccagc ctggaagacg aagctgctgg tcacgtgacc caagctcgca tggtcagtaa
      241 aagcaaagac gggactggaa gcgatgacaa aaaagccaag ggggctgatg gtaaaacgaa
      301 gatcgccaca ccgcggggag cagcccctcc aggccagaag ggccaggcca acgccaccag
      361 gattccagca aaaaccccgc ccgctccaaa gacaccaccc agctctggtg aacctccaaa
      421 atcaggggat cgcagcggct acagcagccc cggctcccca ggcactcccg gcagccgctc
      481 ccgcaccccg tcccttccaa ccccacccac ccgggagccc aagaaggtgg cagtggtccg
      541 tactccaccc aagtcgccgt cttccgccaa gagccgcctg cagacagccc ccgtgcccat
      601 gccagacctg aagaatgtca agtccaagat cggctccact gagaacctga agcaccagcc
      661 gggaggcggg aaggtgcaaa tagtctacaa accagttgac ctgagcaagg tgacctccaa
      721 gtgtggctca ttaggcaaca tccatcataa accaggaggt ggccaggtgg aagtaaaatc
      781 tgagaagctt gacttcaagg acagagtcca gtcgaagatt gggtccctgg acaatatcac
      841 ccacgtccct ggcggaggaa ataaaaagat tgaaacccac aagctgacct tccgcgagaa
      901 cgccaaagcc aagacagacc acggggcgga gatcgtgtac aagtcgccag tggtgtctgg
      961 ggacacgtct ccacggcatc tcagcaatgt ctcctccacc ggcagcatcg acatggtaga
     1021 ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc tccctggcca agcagggttt
     1081 gagctccgga ggggatccac cggtcgccac catggtgagc aagggcgagg agaccacaat
     1141 gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg gagggcaacg tgaatggcca
     1201 cgccttcgtg atcgagggcg agggcgaggg caagccctac gacggcacca acaccatcaa
     1261 cctggaggtg aaggagggag cccccctgcc cttctcctac gacattctga ccaccgcgtt
     1321 cgcctacggc aacagggcct tcaccaagta ccccgacgac atccccaact acttcaagca
     1381 gtccttcccc gagggctact cttgggagcg caccatgacc ttcgaggaca agggcatcgt
     1441 gaaggtgaag tccgacatct ccatggagga ggactccttc atctacgaga tacacctcaa
     1501 gggcgagaac ttccccccca acggccccgt gatgcagaag aagaccaccg gctgggacgc
     1561 ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag ggcgacgtca agcacaagct
     1621 gctgctggag ggcggcggcc accaccgcgt tgacttcaag accatctaca gggccaagaa
     1681 ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc tgaaccacga
     1741 caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg
     1801 catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
     1861 tt
//
