LOCUS       sequence-85905-e        1862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1107..1116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1113..1823
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggatc tgcaagcctc gttgctgtcc actggcccca
       61 atgccagcaa catctccgat ggccaggata atttcacatt ggcggggcca cctcctcgca
      121 caaggagtgt ctcctacatc aacatcatca tgccttcagt gtttggtacc atctgtctcc
      181 tgggcattgt gggaaactcc acagtcattt ttgccgtggt gaagaaatcc aagctgcact
      241 ggtgcagcaa cgtccctgac atcttcatca tcaacctctc tgtggtggat ctgcttttcc
      301 tgctgggcat gcctttcatg atccaccagc tcatgggtaa tggtgtctgg cactttgggg
      361 aaaccatgtg caccctcatc acagccatgg acgccaacag tcagttcacc agcacctaca
      421 tcctgactgc tatggccatt gaccgctact tggccaccgt ccatcccatc tcctccacca
      481 agttccggaa gccctccatg gccaccctgg tgatctgcct cctgtgggct ctctcgttca
      541 ttagcatcac tcctgtgtgg ctctatgcca ggcttatccc cttcccaggg ggtgctgtgg
      601 gctgtggcat ccgcctacca aacccagata ctgatcttta ctggttcact ctgtatcagt
      661 ttttcctggc cttcgccctt ccgtttgtgg tcatcactgc tgcgtacgtg aaaatactac
      721 agcgcatgac gtcttcggtg gccccagcct ctcaacgcag catccggctt cggacaaaga
      781 gggtgacccg cacagccatt gccatctgtc tggtcttctt tgtgtgctgg gcgccctact
      841 acgtgctgca gctgacccag ttgtccatca gccgcccgac cctcacattc gtctacctgt
      901 acaatgcggc tatcagcttg ggctatgcca acagctgcct caatcccttt gtgtacatag
      961 tactctgtga gacctttcga aaacgcttgg tgctgtcggt gaagcccgcg gcccaggggc
     1021 agcttcgcac ggtcagcaat gctcagacag ctgacgagga gaggacagaa agcaaaggca
     1081 ccggaggtag cggggatcca ccggtcgcca ccatggtgag caagggcgag gagaccacaa
     1141 tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac gtgaatggcc
     1201 acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca
     1261 acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt
     1321 tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac tacttcaagc
     1381 agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac aagggcatcg
     1441 tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag atacacctca
     1501 agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc ggctgggacg
     1561 cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc
     1621 tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac agggccaaga
     1681 aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc ctgaaccacg
     1741 acaaggacta caacaaggtg accgtttacg agagcgccgt ggcccgcaac tccaccgacg
     1801 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     1861 tt
//
