LOCUS       sequence-85906-e        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Mito-7
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        108..159
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     110..130
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             188..262
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             296..1006
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gatagcggtt gactcacggg atttccaagt ctccacccat gacgtcaatg ggagttgttt
       61 gcaccaaatc aacgggactt tccaaatgtc gtacaactcc gccccatgac gcaaatgggc
      121 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      181 cgctagcatg tccgtcctga cgccgctgct gctgcggggc ttgacaggct cggcccggcg
      241 gctcccagtg ccgcgcgcca agatccattc gttgggggat ccaccggtcg ccaccatggt
      301 gagcaagggc gaggagacca caatgggcgt aatcaagccc gacatgaaga tcaagctgaa
      361 gatggagggc aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc
      421 ctacgacggc accaacacca tcaacctgga ggtgaaggag ggagcccccc tgcccttctc
      481 ctacgacatt ctgaccaccg cgttcgccta cggcaacagg gccttcacca agtaccccga
      541 cgacatcccc aactacttca agcagtcctt ccccgagggc tactcttggg agcgcaccat
      601 gaccttcgag gacaagggca tcgtgaaggt gaagtccgac atctccatgg aggaggactc
      661 cttcatctac gagatacacc tcaagggcga gaacttcccc cccaacggcc ccgtgatgca
      721 gaagaagacc accggctggg acgcctccac cgagaggatg tacgtgcgcg acggcgtgct
      781 gaagggcgac gtcaagcaca agctgctgct ggagggcggc ggccaccacc gcgttgactt
      841 caagaccatc tacagggcca agaaggcggt gaagctgccc gactatcact ttgtggacca
      901 ccgcatcgag atcctgaacc acgacaagga ctacaacaag gtgaccgttt acgagagcgc
      961 cgtggcccgc aactccaccg acggcatgga cgagctgtac aagtaagcgg ccgcgactct
     1021 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa acctcccaca
     1081 cctccccctg aacctgaaac ataaatgaat gcattgttgt tgtaacttgt ttattgcagc
     1141 ttatatggtt acaataagca tagcatcaca atttcacaaa taagcatttt ttcactgcat
     1201 ctagtgtggt tgtccaactc atcatg
//
