LOCUS       sequence-85909-e        2843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2843)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTFP1-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..744
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagacc
       61 acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat
      121 ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc
      181 atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc
      241 gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc
      301 aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc
      361 atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac
      421 ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg
      481 gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac
      541 aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc
      601 aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac
      661 cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc
      721 gacggcatgg acgagctgta caagtccgga ctcagatctc gagtgagcgc tctggcctcc
      781 cgcgctaccg ccatgcagtc ctccggctcc ttcaactacg cgcgccctaa gcagttcatc
      841 gcggcgcaga acctgggtcc cgcatcgggg ctgcccacgc caacctccag ccccagctcc
      901 tccagcttgc cgtcaccatt gtcccccacg cccaggccct tcggccgcgc gcccgggccg
      961 cccttcgtgg aacccgaggc catgtggggc ccgtcctcgc cctcgccgcc accgccgcca
     1021 cctccggtct tcagcccctc tgcagcctac ccggtgcccg acgtgttccc actgccaccg
     1081 ccgccgccac cgctacctag ctccacctca cactgcgcct cgcccgcccg cttcggcccc
     1141 agccagacgc ccgcagcctt cctcagcgcc ctgctgccct ctcagccacc gcctgtcgct
     1201 gtcaacgccc tggggctgcc caagggagtc acccccgcgg ggttcccaaa gaagtccagt
     1261 agaactgcta gaattgcctc tgatgaggag attcaaggca caaaggatgc tgtcatccaa
     1321 gacctggaac ggaagcttcg cttcaaggag gaccttctga acaatggcca accgaggcta
     1381 acctatgagg aaagaatggc tcgccgcctg cttggagccg acagcgcaaa cgtcttcaac
     1441 atccaggagc cagaggaaac ggcagccaat caggagtaca aagtctctag ctgcgagcag
     1501 aggctgatta gcgagattga atacaggctg gagcgctccc ctgtggatga gtcgggagac
     1561 gaggtgcagg atccagatgt gcctgtggag aacgcaacag ctcccttctt tgagatgaag
     1621 ctgaaacact acaagatctt tgaggggatg ccggtgactt tcacgtgtcg agtggctggg
     1681 aatccaaagc caaagatcta ttggtttaaa gatgggaagc agatttctcc gaagagcgat
     1741 cactacacca ttcagagaga ccttgatggg acctgctctc tccacaccac ggcctctacc
     1801 ctagacgacg atgggaacta caccatcatg gctgccaacc ctcagggtcg cgtcagttgt
     1861 acaggaaggc taatggtaca ggctgtcaac caaagaggcc gcagtccccg ctctccctca
     1921 ggccatcctc atgccagaag gcctcgctct cgatcacggg acagtggaga tgaaaacgag
     1981 cccattcagg agcgattctt cagacctcac ttcctgcagg ctcctggaga cctgaccgtt
     2041 caggaaggca agctctgcag gatggactgc aaggtcagtg ggttaccaac cccagatctc
     2101 agctggcaac tagacggaaa gcccatacgc cccgacagtg ctcacaagat gctggtccgt
     2161 gagaatgggg tccactccct cattatagag ccagtcacgt cccgggacgc cggcatctac
     2221 acatgtattg ccaccaacag agcaggacag aactcgttta acctggagct tgtggttgct
     2281 gctaaggaag cacacaaggc ccctgtgttt atggagaagc tacagaacac gggggttgct
     2341 gatggatacc cagtgcggct agaatgccgt gtctccggag tgccgccacc tcagatattt
     2401 tggaagaaag aaaatgaatc gctcactcat agcactgagc gagtaagcat gcaccaggat
     2461 aatcatggct acatctgcct gctcatccag ggagccacaa aggaagacgc tgggtggtac
     2521 actgtgtctg ccaagaacga agcaggcatt gtgtcctgca ctgcccggct ggatgtctac
     2581 acccagtggc accagcagcc acagaccacc aagccaaaaa aagtacggcc ctcggccagt
     2641 cgctacgcag cactttcgga ccagggacta gacatcaaag ccgctttcca acctgaagcc
     2701 agcccatctc acctgactct gaacagcggc ttggtagaaa gtgaagacct gtgagaattc
     2761 tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag
     2821 ccataccaca ttgtagaggt tag
//
