LOCUS       sequence-85911-e        1708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1708)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTFP1-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..50
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             78..98
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..818
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 agacgtcaga tccgctagcc accatggatc caaaaaagaa gagaaaggta gatccaaaaa
       61 agaagagaaa ggtagatcca aaaaagaaga gaaaggtacc ggtcgccacc atggtgagca
      121 agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg
      181 agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg
      241 acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg
      301 acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac cccgacgaca
      361 tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct
      421 tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
      481 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga
      541 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
      601 gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
      661 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
      721 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg
      781 cccgcaactc caccgacggc atggacgagc tgtacaagtc cggagaaggg caagggcaag
      841 ggcaagggcc gggccgcggc tacgcgtatc gatccatgtt cgaggcgcgc ctggtccagg
      901 gctccatcct caagaaggtg ttggaggcac tcaaggacct catcaacgag gcctgctggg
      961 atattagctc cagcggtgta aacctgcaga gcatggactc gtcccacgtc tctttggtgc
     1021 agctcaccct gcggtctgag ggcttcgaca cctaccgctg cgaccgcaac ctggccatgg
     1081 gcgtgaacct caccagtatg tccaaaatac taaaatgcgc cggcaatgaa gatatcatta
     1141 cactaagggc cgaagataac gcggatacct tggcgctagt atttgaagca ccaaaccagg
     1201 agaaagtttc agactatgaa atgaagttga tggatttaga tgttgaacaa cttggaattc
     1261 cagaacagga gtacagctgt gtagtaaaga tgccttctgg tgaatttgca cgtatatgcc
     1321 gagatctcag ccatattgga gatgctgttg taatttcctg tgcaaaagac ggagtgaaat
     1381 tttctgcaag tggagaactt ggaaatggaa acattaaatt gtcacagaca agtaatgtcg
     1441 ataaagagga ggaagctgtt accatagaga tgaatgaacc agttcaacta acttttgcac
     1501 tgaggtacct gaacttcttt acaaaagcca ctccactctc ttcaacggtg acactcagta
     1561 tgtctgcaga tgtacccctt gttgtagagt ataaaattgc ggatatggga cacttaaaat
     1621 actacttggc tcccaagatc gaggatgaag aaggatctta gggatccacc ggatctagat
     1681 aactgatcat aatcagccat accacatt
//
