LOCUS       sequence-85913-e        1466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaccaca
       61 atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa cgtgaatggc
      121 cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac caacaccatc
      181 aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct gaccaccgcg
      241 ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa ctacttcaag
      301 cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga caagggcatc
      361 gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga gatacacctc
      421 aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac cggctgggac
      481 gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt caagcacaag
      541 ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta cagggccaag
      601 aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat cctgaaccac
      661 gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa ctccaccgac
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgtcgca gacggccatg
      781 tccgaaacct acgatttttt gtttaagttc ttggttattg gaaatgcagg aactggcaaa
      841 tcttgcttac ttcatcagtt tattgaaaaa aaattcaaag atgactcaaa tcatacaata
      901 ggagtggaat ttggttcaaa gataataaat gttggtggta aatatgtaaa gttacaaata
      961 tgggatacag caggacaaga acgattcagg tccgtgacga gaagttatta ccgaggcgcg
     1021 gccggggctc tcctcgtcta tgatatcacc agccgagaaa cctacaatgc gcttactaat
     1081 tggttaacag atgcccgaat gctggcgagc cagaacattg tgatcatcct ttgtggaaac
     1141 aagaaggacc tggatgcaga tcgtgaagtt accttcttag aagcctccag atttgctcaa
     1201 gaaaatgagc tgatgttttt ggaaacaagt gcgctcacag gggagaatgt agaagaggct
     1261 tttgtacagt gtgcaagaaa aatacttaac aaaatcgaat caggtgagct ggacccagaa
     1321 agaatgggct caggtattca gtacggagat gctgccttga gacagctgag gtcaccgcgg
     1381 cgcgcacagg ccccgaacgc tcaggagtgt ggttgttagg gatccaccgg atctagataa
     1441 ctgatcataa tcagccatac cacatt
//
