LOCUS       sequence-85915-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1381..2091
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatggc cactgttacc
       61 tatccttcat ctgagcctac gaccttggac cctgggaacg catcctcgac ctggcccctg
      121 gataccaccc tggggaacac atccgctggc gctagcctga caggcctggc tgtcagtggc
      181 atcttgatct ctctggtgta cctggtggtg tgcgtggtgg gtttgctggg caactcgctg
      241 gtgatctacg tggtcctgcg gcacacgtcc agcccatcag tgaccagtgt ctatatcctc
      301 aacctggctc tggctgatga gctcttcatg ctagggctac ccttcctggc tgctcagaac
      361 gccctgtcct actggccctt cggatctctc atgtgccgtc tggtcatggc cgtggatggc
      421 atcaaccagt tcaccagcat cttctgcctc accgtcatga gtgtggaccg ctatctggct
      481 gtggtgcacc ccacacgctc agcccgctgg cgcacggcac cagtggctcg cacggtcagt
      541 gcagctgtct gggtggcctc ggctgtggtg gtgctgcctg tggttgtgtt ctcaggagtg
      601 ccccggggca tgagcacgtg ccacatgcag tggccagagc cagcggctgc ctggcgaaca
      661 gccttcatca tctacacggc cgcactgggc ttctttgggc ccctgctggt catctgcttg
      721 tgctacttgc tcattgtggt aaaggtgcgg tcgaccaccc ggcgggtgcg ggcgccctcg
      781 tgtcagtggg tacaggcacc cgcatgccag cggcgacgcc gctctgagcg cagggtcaca
      841 cgcatggtgg tggccgtggt ggcactcttc gtcctctgct ggatgccttt ctatctgctc
      901 aacatcgtca atgtggtgtg cccgctgccg gaggagcccg ccttcttcgg cctctacttc
      961 ctggtggtgg cgctgcccta cgccaacagc tgcgcaaacc ccatcctcta cggcttcctc
     1021 tcctaccgct tcaagcaggg ctttcgcagg atcctgctaa gaccatcacg tcgcattcgg
     1081 agccaggagc cagggtcggg acctccagag aagactgaag aggaggagga tgaagaagaa
     1141 gaagagagaa gggaagagga ggagcggagg atgcagagag ggcaggagat gaacgggagg
     1201 ctcagtcaga tcgcacaggc tggcactagt ggacaacagc cacggccctg cacagggact
     1261 gctaaggagc agcagcttct gccccaggag gccacagctg gggacaaggc cagcacactg
     1321 agccatctgg gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     1381 atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag
     1441 ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc
     1501 aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc
     1561 ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac
     1621 cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc
     1681 accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag
     1741 gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg
     1801 atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc
     1861 gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt
     1921 gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg
     1981 gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag
     2041 agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagta agcggccgcg
     2101 actctagatc ataatcagcc ataccacatt
//
