LOCUS       sequence-85917-e        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 462, 
            Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      483..492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..1199
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggtgg gtcggaacag cgccatcgcc gccggtgtat
       61 gcggggccct tttcattggg tactgcatct acttcgaccg caaaagacga agtgacccca
      121 acttcaagaa caggcttcga gaacgaagaa agaaacagaa gcttgccaag gagagagctg
      181 ggctttccaa gttacctgac cttaaagatg ctgaagctgt tcagaagttc ttccttgaag
      241 aaatacagct tggtgaagag ttactagctc aaggtgaata tgagaagggc gtagaccatc
      301 tgacaaatgc aattgctgtg tgtggacagc cacagcagtt actgcaggtc ttacagcaaa
      361 ctcttccacc accagtgttc cagatgcttc tgactaagct cccaacaatt agtcagagaa
      421 ttgtaagtgc tcagagcttg gctgaagatg atgtggaagg cggtagcggg gatccaccgg
      481 tcgccaccat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga
      541 agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg
      601 gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc
      661 ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca
      721 ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt
      781 gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca
      841 tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg
      901 gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc
      961 gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
     1021 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
     1081 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
     1141 tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagtaag
     1201 cggccgcgac tctagatcat aatcagccat accacattt
//
