LOCUS       sequence-85920-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctatg agcgagacgg tcatctgttc
      781 cagccgggct actgtgatgc tttatgatga cagcaacaag cgatggctcc ctgctggcac
      841 tggtccccag gccttcagcc gcgtccagat ataccacaac cccactgcta actccttccg
      901 agttgttggc cgcaagatgc agccggatca gcaggtggtt atcaactgtg ccatcattcg
      961 gggtgtcaag tacaatcagg ccactcccat cttccatcag tggcgagatg cccgccaggt
     1021 ctggggcctc aacttcggca gcaaggagga cgccatacag tttgcaacag gcatggccaa
     1081 cgccctagag gccttggaag gaggtgggcc tcccccagcc ccagcacccc ctgcctggtc
     1141 tgcccagaat ggtccctccc cagaggagct ggaacaacag aaaaggcagc cggagcatat
     1201 ggagcgccgg gtctccaatg caggaggccc acctgctccc ccagctgggg gccctcctcc
     1261 acctccagga cctccccctc ctccaggtcc ccccccaccc ccaggtctgc cctcctcagg
     1321 ggtatctggg gcaggtcatg gagcaggggc agccccaccc cctgcacccc cactccctac
     1381 aacacagggc cccaatagtg ggggttccgg ggccccaggc ctggctgctg ccattgctgg
     1441 agccaaactc aggaaagtga gcaagcagga ggaggcctct ggggggcccc tggcccccaa
     1501 agctgagaac agtcgaagca ctggtggggg gcttatggaa gagatgaacg ccatgctggc
     1561 ccggagaaga aaagccacac aggttgggga gaagcccccc aaagacgagt cagccagtga
     1621 ggagtcagag gcccgactcc ctgcccagag tgaacctgtg agaagaccct gggagaagaa
     1681 cagcacaacc ttgccaagga tgaagtcgtc ctcttctgtg actacctccg aggcccaccc
     1741 ctcgacgccc tgctccagtg atgactccga cttggagagg gtgaagcagg agcttctgga
     1801 agaggtgcgg aaggagctac agaaaatgaa agaggaaatc atcgaagtct ttgtccagga
     1861 gctgaggaag cggggttctc cttgagcggc cgcgactcta gatcataatc agccata
//
