LOCUS       sequence-85925-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER Transport, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-VSVG-16
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             220..930
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ggttggttca gtagttggaa gagctctatt gcctcttttt tctttatcat agggttaatc
       61 attggactat tcttggttct ccgagttggt atttatcttt gcattaaatt aaagcacacc
      121 aagaaaagac agatttatac agacatagag atgaaccgac ttggaaacag aattctgcag
      181 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
      241 accacaatgg gcgtaatcaa gcccgacatg aagatcaagc tgaagatgga gggcaacgtg
      301 aatggccacg ccttcgtgat cgagggcgag ggcgagggca agccctacga cggcaccaac
      361 accatcaacc tggaggtgaa ggagggagcc cccctgccct tctcctacga cattctgacc
      421 accgcgttcg cctacggcaa cagggccttc accaagtacc ccgacgacat ccccaactac
      481 ttcaagcagt ccttccccga gggctactct tgggagcgca ccatgacctt cgaggacaag
      541 ggcatcgtga aggtgaagtc cgacatctcc atggaggagg actccttcat ctacgagata
      601 cacctcaagg gcgagaactt cccccccaac ggccccgtga tgcagaagaa gaccaccggc
      661 tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg tgctgaaggg cgacgtcaag
      721 cacaagctgc tgctggaggg cggcggccac caccgcgttg acttcaagac catctacagg
      781 gccaagaagg cggtgaagct gcccgactat cactttgtgg accaccgcat cgagatcctg
      841 aaccacgaca aggactacaa caaggtgacc gtttacgaga gcgccgtggc ccgcaactcc
      901 accgacggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
      961 taccacat
//
