LOCUS       sequence-85926-e        6029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     RBS             2436..2444
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagac cacaatgggc
       61 gtaatcaagc ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa tggccacgcc
      121 ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg gcaccaacac catcaacctg
      181 gaggtgaagg agggagcccc cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc
      241 tacggcaaca gggccttcac caagtacccc gacgacatcc ccaactactt caagcagtcc
      301 ttccccgagg gctactcttg ggagcgcacc atgaccttcg aggacaaggg catcgtgaag
      361 gtgaagtccg acatctccat ggaggaggac tccttcatct acgagataca cctcaagggc
      421 gagaacttcc cccccaacgg ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc
      481 accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca caagctgctg
      541 ctggagggcg gcggccacca ccgcgttgac ttcaagacca tctacagggc caagaaggcg
      601 gtgaagctgc ccgactatca ctttgtggac caccgcatcg agatcctgaa ccacgacaag
      661 gactacaaca aggtgaccgt ttacgagagc gccgtggccc gcaactccac cgacggcatg
      721 gacgagctgt acaagtccgg aagtgctggg agcgcaagcg gaagcggagg ctccggtgag
      781 gaaacagcta tatgggaaca acatacagtg acgcttcaca gggctcctgg atttggattt
      841 ggaattgcaa tatctggtgg acgagataat cctcattttc agagtgggga aacgtcaata
      901 gtgatttcag atgtgctgaa aggaggacca gctgaaggac agctacagga aaatgaccga
      961 gttgcaatgg ttaacggagt ttcaatggat aatgttgaac atgcttttgc tgttcagcaa
     1021 ctaaggaaaa gtgggaaaaa tgcaaaaatt acaattagaa ggaagaagaa agttcaaata
     1081 ccagtaagtc gtcctgatcc tgaaccagta tctgataatg aagaagatag ttatgatgag
     1141 gaaatacatg atccaagaag tggccggagt ggtgtggtta acagaaggag tgagaagatt
     1201 tggccgaggg atagaagtgc aagtagagag aggagcttgt ccccgcggtc agacaggcgg
     1261 tcagtggctt ccagccagcc tgctaaacct actaaagtca cactggtgaa atcccggaaa
     1321 aatgaagaat atggtcttcg attggcaagc catatatttg ttaaggaaat ttcacaagat
     1381 agtttggcag caagagatgg caatattcaa gaaggtgatg ttgtattgaa gataaatggt
     1441 actgtgacag aaaatatgtc attgacagat gcaaagacat tgatagaaag gtctaaaggc
     1501 aaattaaaaa tggtagttca aagagatgaa cgggctacgc tattgaatgt ccctgatctt
     1561 tctgacagca tccactctgc taatgcctct gagagagacg acatttcaga aattcagtca
     1621 ctggcatcag atcattctgg tcgatcacac gataggcctc cccgccgcag ccggtcacga
     1681 tctcctgacc agcggtcaga gccttctgat cattccaggc actcgccgca gcagccaagc
     1741 aatggcagtc tgcggagtag agatgaagag agaatttcta aacctggggc tgtctcaact
     1801 cctgtaaagc atgctgatga tcacacacct aaaacagtgg aagaagttac agttgaaaga
     1861 aatgagaaac aaacaccttc tcttccagaa ccaaagcctg tgtatgccca agttggcaac
     1921 cagatgtgga tttacctgtc agtccatctg atggtgtcct acctaattca actcatgaag
     1981 atgggatttc ttcggcccag catgaaattg gtaaaattca gaaaaggaga tagtgtgggt
     2041 ttgcggctgg ctggtggaaa tgatgttgga atatttgtag ctggcgttct agaagatagc
     2101 cctgcagcca aggaaggctt agaggaaggt gatcaaattc tcagggtaaa caacgtagat
     2161 tttacaaata tcataagaga agaagccgtc cttttcctgc ttgacctccc taaaggagaa
     2221 gaagtgacca tattggctca gaagaagaag gatgtttatc gtcgcattgt agaatcagat
     2281 gtaggagatt ctttctatat tagaacccat tttgaatatg aaaaggaatc tccctatgga
     2341 cttagtttta acaaaggaga ggtgttccgt gctgtggata ccttgtacaa tggaaaactg
     2401 ggctcttggc ttgctattcg aattggtaaa aatcataagg aggtagaacg aggcatcatc
     2461 cctaataaga acagagctga gcagctcgcc agtgtacagt atacacttcc aaaaacagca
     2521 ggcggagacc gtgctgactt ctggagattc agaggtcttc gcagctccaa gagaaatctt
     2581 cgaaaaagca gagaggattt gtccgctcag cctgttcaaa caaagtttcc agcttatgaa
     2641 agagtggttc ttcgagaagc tggatttctg aggcctgtaa ccatttttgg accaatagct
     2701 gatgttgcca gagaaaagct ggcaagagaa gaaccagata tttatcaaat tgcaaagagt
     2761 gaaccacgag acgctggaac tgaccaacgt agctctggct atattcgcct gcatacaata
     2821 aagcaaatca tagatcaaga caaacatgct ttattagatg taacaccaaa tgcagttgat
     2881 cgtcttaact atgcccagtg gtatccaatt gttgtatttc ttaaccctga ttctaagcaa
     2941 ggagtaaaaa caatgagaat gaggttatgt ccagaatctc ggaaaagtgc caggaagtta
     3001 tacgagcgat ctcataaact tgctaaaaat aatcaccatc tttttacaac tacaattaac
     3061 ttaaattcaa tgaatgatgg ttggtatggt gcgctgaaag aagcagttca acaacagcaa
     3121 aaccagctgg tatgggtttc cgagggaaag gcggatggtg ctacaagtga tgaccttgat
     3181 ttgcatgatg atcgtctgtc ctacctgtca gctccaggta gtgaatactc aatgtatagc
     3241 acggacagta gacacacttc tgactatgaa gacacagaca cagaaggcgg ggcctacact
     3301 gatcaagaac tagatgaaac tcttaatgat gaggttggga ctccaccgga gtctgccatt
     3361 acacggtcct ctgagcctgt aagagaggac tcctctggaa tgcatcatga aaaccaaaca
     3421 tatcctcctt actcaccaca agcgcagcca caaccaattc atagaataga ctcccctgga
     3481 tttaagccag cctctcaaca gaaagcagaa gcttcatctc cagtccctta cctttcgcct
     3541 gaaacaaacc cagcatcatc aacctctgct gttaatcata atgtaaattt aactaatgtc
     3601 agactggagg agcccacccc agctccttcc acctcttact caccacaagc tgattcttta
     3661 agaacaccaa gtactgaggc agctcacata atgctaagag atcaagaacc atcattgtcg
     3721 tcgcatgtag atccaacaaa ggtgtataga aaggacccat atcccgagga aatgatgagg
     3781 cagaaccatg ttttgaaaca gccagccgtt agtcacccag ggcacaggcc agacaaagag
     3841 cctaatctga cctatgaacc ccaactccca tacgtagaga aacaagccag cagagacctg
     3901 gagcagccca catacagata cgagtcctca agctatacgg accagttttc tcgaaactat
     3961 gaacatcgtc tgcgatacga agatcgcgtc cccatgtatg aagaacagtg gtcatattat
     4021 gatgacaaac agccctaccc atctcggcca ccttttgata atcagcactc tcaagacctt
     4081 gactccagac agcatcccga agagtcctca gaacgagggt actttccacg ttttgaagag
     4141 ccagcccctc tgtcttacga cagcagacca cgttacgaac aggcacctag agcatccgcc
     4201 ctgcggcacg aagagcagcc agctcctggg tatgacacac atggtagact cagaccggaa
     4261 gcccagcccc acccttcagc agggcccaag cctgcagagt ccaagcagta ttttgagcaa
     4321 tattcacgca gttacgagca agtaccaccc caaggattta cctctagagc aggtcatttt
     4381 gagcctctcc atggtgctgc agctgtccct ccgctgatac cttcatctca gcataagcca
     4441 gaagctctgc cttcaaacac caaaccactg cctccacccc caactcaaac cgaagaagag
     4501 gaagatccag caatgaagcc acagtctgta ctcaccagag ttaagatgtt tgaaaacaaa
     4561 aggtctgcat ccttagagac caagaaggat gtaaatgaca ctggcagttt taagcctcca
     4621 gaagtagcat ctaaaccttc aggtgctccc atcattggtc ccaaacccac ttctcagaat
     4681 caattcagtg aacatgacaa aactctgtac aggattccag aacctcaaaa acctcaactg
     4741 aagccacctg aagatattgt tcggtccaat cattatgacc ctgaagaaga tgaagaatat
     4801 tatcgaaaac agctgtcata ctttgaccga agaagttttg agaataagcc tcctgcacac
     4861 attgctgcca gccatctctc cgagcctgca aagccagctc attctcagaa tcaatcaaat
     4921 ttttctagtt attcttcaaa gggaaagcct cctgaagctg atggtgtgga tagatcattt
     4981 ggcgagaaac gctatgaacc catccaggcc actccccctc ctcctccatt gccctcgcag
     5041 tatgcccagc catctcagcc tgtcaccagc gcgtctctcc acatacattc taagggagca
     5101 catggtgaag gtaattcagt gtcattggat tttcagaatt ccttagtgtc caaaccagac
     5161 ccacctccat ctcagaataa gccagcaact ttcagaccac caaaccgaga agatactgct
     5221 caggcagctt tctatcccca gaaaagtttt ccagataaag ccccagttaa tggaactgaa
     5281 cagactcaga aaacagtcac tccagcatac aatcgattca caccaaaacc atatacaagt
     5341 tctgcccgac catttgaacg caagtttgaa agtcctaaat tcaatcacaa tcttctgcca
     5401 agtgaaactg cacataaacc tgacttgtct tcaaaaactc ccacttctcc aaaaactctt
     5461 gtgaaatcgc acagtttggc acagcctcct gagtttgaca gtggagttga aactttctct
     5521 atccatgcag agaagcctaa atatcaaata aataatatca gcacagtgcc taaagctatt
     5581 cctgtgagtc cttcagctgt ggaagaggat gaagatgaag atggtcatac tgtggtggcc
     5641 acagcccgag gcatatttaa cagcaatggg ggcgtgctga gttccataga aactggtgtt
     5701 agtataatta tccctcaagg agccattccc gaaggagttg agcaggaaat ctatttcaag
     5761 gtctgccggg acaacagcat ccttccacct ttagataaag agaaaggtga aacactgctg
     5821 agtcctttgg tgatgtgtgg tccccatggc ctcaagttcc tgaagcctgt ggagctgcgc
     5881 ttaccacact gtgatcctaa aacctggcaa aacaagtgtc ttcccggaga tccaaattat
     5941 ctcgttggag caaactgtgt ttctgtcctt attgaccact tttaaggatc caccggatct
     6001 agataactga tcataatcag ccataccac
//
