LOCUS       sequence-85928-e        1955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Filaments, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1955)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggactcagat ctggcagcgg tggaggcagc gcatccggcg
      781 gaagcggaag cgatgatgat atcgccgcgc tcgtcgtcga caacggctcc ggcatgtgca
      841 aggccggctt cgcgggcgac gatgcccccc gggccgtctt cccctccatc gtggggcgcc
      901 ccaggcacca gggcgtgatg gtgggcatgg gtcagaagga ttcctatgtg ggcgacgagg
      961 cccagagcaa gagaggcatc ctcaccctga agtaccccat cgagcacggc atcgtcacca
     1021 actgggacga catggagaaa atctggcacc acaccttcta caatgagctg cgtgtggctc
     1081 ccgaggagca ccccgtgctg ctgaccgagg cccccctgaa ccccaaggcc aaccgcgaga
     1141 agatgaccca gatcatgttt gagaccttca acaccccagc catgtacgtt gctatccagg
     1201 ctgtgctatc cctgtacgcc tctggccgta ccactggcat cgtgatggac tccggtgacg
     1261 gggtcaccca cactgtgccc atctacgagg ggtatgccct cccccatgcc atcctgcgtc
     1321 tggacctggc tggccgggac ctgactgact acctcatgaa gatcctcacc gagcgcggct
     1381 acagcttcac caccacggcc gagcgggaaa tcgtgcgtga cattaaggag aagctgtgct
     1441 acgtcgccct ggacttcgag caagagatgg ccacggctgc ttccagctcc tccctggaga
     1501 agagctacga gctgcctgac ggccaggtca tcaccattgg caatgagcgg ttccgctgcc
     1561 ctgaggcact cttccagcct tccttcctgg gcatggagtc ctgtggcatc cacgaaacta
     1621 ccttcaactc catcatgaag tgtgacgtgg acatccgcaa agacctgtac gccaacacag
     1681 tgctgtctgg cggcaccacc atgtaccctg gcattgccga caggatgcag aaggagatca
     1741 ctgccctggc acccagcaca atgaagatca agatcattgc tcctcctgag cgcaagtact
     1801 ccgtgtggat cggcggctcc atcctggcct cgctgtccac cttccagcag atgtggatca
     1861 gcaagcagga gtatgacgag tccggcccct ccatcgtcca ccgcaaatgc ttctagggat
     1921 ccaccggatc tagataactg atcataatca gccat
//
