LOCUS       sequence-85929-e        2382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2382)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ALK1-C-18
FEATURES             Location/Qualifiers
     source          1..2382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..49,50..52,53..763)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(92..113)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(353..372)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     700..721
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccaatttta gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      181 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      241 gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      481 ctacatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      541 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
      601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      661 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      721 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg
      781 agctcaagct tcgaattcag ggtctggaag tggtagcacc ttgggctccc ccaggaaagg
      841 ccttctgatg ctgctgatgg ccttggtgac ccagggagac cctgtgaagc cgtctcgggg
      901 cccgctggtg acctgcacgt gtgagagccc acattgcaag gggcctacct gccggggggc
      961 ctggtgcaca gtagtgctgg tgcgggagga ggggaggcac ccccaggaac atcggggctg
     1021 cgggaacttg cacagggagc tctgcagggg gcgccccacc gagttcgtca accactactg
     1081 ctgcgacagc cacctctgca accacaacgt gtccctggtg ctggaggcca cccaacctcc
     1141 ttcggagcag ccgggaacag atggccagct ggccctgatc ctgggccccg tgctggcctt
     1201 gctggccctg gtggccctgg gtgtcctggg cctgtggcat gtccgacgga ggcaggagaa
     1261 gcagcgtggc ctgcacagcg agctgggaga gtccagtctc atcctgaaag catctgagca
     1321 gggcgacagc atgttggggg acctcctgga cagtgactgc accacaggga gtggctcagg
     1381 gctccccttc ctggtgcaga ggacagtggc acggcaggtt gccttggtgg agtgtgtggg
     1441 aaaaggccgc tatggcgaag tgtggcgggg cttgtggcac ggtgagagtg tggccgtcaa
     1501 gatcttctcc tcgagggatg aacagtcctg gttccgggag actgagatct ataacacagt
     1561 gttgctcaga cacgacaaca tcctaggctt catcgcctca gacatgacct cccgcaactc
     1621 gagcacgcag ctgtggctca tcacgcacta ccacgagcac ggctccctct acgactttct
     1681 gcagagacag acgctggagc cccatctggc tctgaggcta gctgtgtccg cggcatgcgg
     1741 cctggcgcac ctgcacgtgg agatcttcgg tacacagggc aaaccagcca ttgcccaccg
     1801 cgacttcaag agccgcaatg tgctggtcaa gagcaacctg cagtgttgca tcgccgacct
     1861 gggcctggct gtgatgcact cacagggcag cgattacctg gacatcggca acaacccgag
     1921 agtgggcacc aagcggtaca tggcacccga ggtgctggac gagcagatcc gcacggactg
     1981 ctttgagtcc tacaagtgga ctgacatctg ggcctttggc ctggtgctgt gggagattgc
     2041 ccgccggacc atcgtgaatg gcatcgtgga ggactataga ccacccttct atgatgtggt
     2101 gcccaatgac cccagctttg aggacatgaa gaaggtggtg tgtgtggatc agcagacccc
     2161 caccatccct aaccggctgg ctgcagaccc ggtcctctca ggcctagctc agatgatgcg
     2221 ggagtgctgg tacccaaacc cctctgcccg actcaccgcg ctgcggatca agaagacact
     2281 acaaaaaatt agcaacagtc cagagaagcc taaagtgatt caatagggat ccaccggatc
     2341 tagataactg atcataatca gccataccac atgagagtag cc
//
