LOCUS       sequence-85930-e        2366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2366)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ALK1-N-13
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1603..1612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1609..1611,1612..1614,1615..2328)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1654..1675)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1915..1934)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2262..2283
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcaccgcc
       61 atgaccttgg gctcccccag gaaaggcctt ctgatgctgc tgatggcctt ggtgacccag
      121 ggagaccctg tgaagccgtc tcggggcccg ctggtgacct gcacgtgtga gagcccacat
      181 tgcaaggggc ctacctgccg gggggcctgg tgcacagtag tgctggtgcg ggaggagggg
      241 aggcaccccc aggaacatcg gggctgcggg aacttgcaca gggagctctg cagggggcgc
      301 cccaccgagt tcgtcaacca ctactgctgc gacagccacc tctgcaacca caacgtgtcc
      361 ctggtgctgg aggccaccca acctccttcg gagcagccgg gaacagatgg ccagctggcc
      421 ctgatcctgg gccccgtgct ggccttgctg gccctggtgg ccctgggtgt cctgggcctg
      481 tggcatgtcc gacggaggca ggagaagcag cgtggcctgc acagcgagct gggagagtcc
      541 agtctcatcc tgaaagcatc tgagcagggc gacagcatgt tgggggacct cctggacagt
      601 gactgcacca cagggagtgg ctcagggctc cccttcctgg tgcagaggac agtggcacgg
      661 caggttgcct tggtggagtg tgtgggaaaa ggccgctatg gcgaagtgtg gcggggcttg
      721 tggcacggtg agagtgtggc cgtcaagatc ttctcctcga gggatgaaca gtcctggttc
      781 cgggagactg agatctataa cacagtgttg ctcagacacg acaacatcct aggcttcatc
      841 gcctcagaca tgacctcccg caactcgagc acgcagctgt ggctcatcac gcactaccac
      901 gagcacggct ccctctacga ctttctgcag agacagacgc tggagcccca tctggctctg
      961 aggctagctg tgtccgcggc atgcggcctg gcgcacctgc acgtggagat cttcggtaca
     1021 cagggcaaac cagccattgc ccaccgcgac ttcaagagcc gcaatgtgct ggtcaagagc
     1081 aacctgcagt gttgcatcgc cgacctgggc ctggctgtga tgcactcaca gggcagcgat
     1141 tacctggaca tcggcaacaa cccgagagtg ggcaccaagc ggtacatggc acccgaggtg
     1201 ctggacgagc agatccgcac ggactgcttt gagtcctaca agtggactga catctgggcc
     1261 tttggcctgg tgctgtggga gattgcccgc cggaccatcg tgaatggcat cgtggaggac
     1321 tatagaccac ccttctatga tgtggtgccc aatgacccca gctttgagga catgaagaag
     1381 gtggtgtgtg tggatcagca gacccccacc atccctaacc ggctggctgc agacccggtc
     1441 ctctcaggcc tagctcagat gatgcgggag tgctggtacc caaacccctc tgcccgactc
     1501 accgcgctgc ggatcaagaa gacactacaa aaaattagca acagtccaga gaagcctaaa
     1561 gtgattcaag gcagcgcagg cggaagcggg gatccaccgg tcgccaccat ggtgagcaag
     1621 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1681 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1741 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1801 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1861 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1921 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1981 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2041 aactacatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     2101 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     2161 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2221 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2281 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     2341 tctagatcat aatcagccat accaca
//
