LOCUS       sequence-85934-e        1779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1779)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG3-N-14
FEATURES             Location/Qualifiers
     source          1..1779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1016..1025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1022..1024,1025..1027,1028..1741)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1067..1088)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1328..1347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1675..1696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggact cagatctcga ggccaccatg cagaatgtga tcaacacggt
       61 gaagggaaag gctctggaag tggccgagta cctgaccccg gtcctcaagg aatcaaaatt
      121 taaggaaacg ggtgtaatca ctccagaaga gtttgtggca gctggagatc acttagtcca
      181 ccactgtcca acatggcaat gggctacagg ggaagaattg aaagtgaagg catatcttcc
      241 gacagacaaa caatttttgg taaccaaaaa tgttccatgc tacaagcggt gtaaacagat
      301 ggagtattcg gatgaattgg aagctatcat tgaagaagat gatggtgatg ggggatgggt
      361 agatacatat cacaacacag gtattacagg aattactgaa gcagttaagg agattacact
      421 ggaaagcaag gacagtataa aactccaaga ttgctcagca ctgtgtgatg aagaagacga
      481 ggaagatgaa ggggaagctg cagacatgga agaatatgaa gagagtggat tgttggaaac
      541 agatgaggct accctagaca caaggaaaat agtggaagcc tgcaaagcta aggctgacgc
      601 tggaggtgaa gatgctattt tacaaacgag aacatacgat ctgtacatca cttacgacaa
      661 atattaccag acaccacggc tatggttgtt tggctatgat gagcaacggc agcctttaac
      721 agttgagcac atgtatgaag acatcagtca agatcatgtg aagaaaacag tgaccattga
      781 aaaccatcct catctcccac cacctcctat gtgttcagtt cacccatgca ggcatgctga
      841 agtgatgaag aaaattattg agacagttgc agaaggcggg ggagagcttg gtgttcatat
      901 gtatctttta atttttttga aatttgttca agctgtcatt ccaacaatag aatatgacta
      961 cacaagacac ttcacaatgg gcagcgcatc cggcggaagc ggaagcggac cggtcgccac
     1021 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1081 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1141 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1201 cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     1261 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1321 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1381 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1441 caagctggag tacaactaca tcagcgacaa cgtctatatc accgccgaca agcagaagaa
     1501 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     1561 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1621 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1681 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     1741 aagcggccgc gactctagat cataatcagc cataccaca
//
