LOCUS       sequence-85935-e        1696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1696)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG5-C-18
FEATURES             Location/Qualifiers
     source          1..1696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(45..47,48..50,51..761)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     698..719
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt
      241 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      481 acatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
      541 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
      601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      661 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag
      781 gaagcggcgg atctgggtcc ggcagctccg gcagcacaga tgacaaagat gtgcttcgag
      841 atgtgtggtt tggacgaatt ccaacttgtt tcacgctata tcaggatgag ataactgaaa
      901 gggaagcaga accatactat ttgcttttgc caagagtaag ttatttgacg ttggtgactg
      961 acaaagtgaa aaagcacttt cagaaggtta tgagacaaga agacattagt gagatatggt
     1021 ttgaatatga aggcacacca ctgaaatggc attatccaat tggtttgcta tttgatcttc
     1081 ttgcatcaag ttcagctctt ccttggaaca tcacagtaca ttttaagagt tttccagaaa
     1141 aagaccttct gcactgtcca tctaaggatg caattgaagc tcattttatg tcatgtatga
     1201 aagaagctga tgctttaaaa cataaaagtc aagtaatcaa tgaaatgcag aaaaaagatc
     1261 acaagcaact ctggatggga ttgcaaaatg acagatttga ccagttttgg gccatcaatc
     1321 ggaaactcat ggaatatcct gcagaagaaa atggatttcg ttatatcccc tttagaatat
     1381 atcagacaac gactgaaaga cctttcattc agaagctgtt tcgtcctgtg gctgcagatg
     1441 gacagttgca cacactagga gatctcctca aagaagtttg tccttctgct attgatcctg
     1501 aagatgggga aaaaaagaat caagtgatga ttcatggaat tgagccaatg ttggaaacac
     1561 ctctgcagtg gctgagtgaa catctgagct acccggataa ttttcttcat attagtatca
     1621 tcccacagcc aacagattga ggtaccgcgg gcccgggatc caccggatct agataactga
     1681 tcataatcag ccatac
//
