LOCUS       sequence-85936-e        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG5-N-14
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(902..904,905..907,908..1621)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(947..968)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1208..1227)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1555..1576
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagac cgccatgaca gatgacaaag atgtgcttcg
       61 agatgtgtgg tttggacgaa ttccaacttg tttcacgcta tatcaggatg agataactga
      121 aagggaagca gaaccatact atttgctttt gccaagagta agttatttga cgttggtgac
      181 tgacaaagtg aaaaagcact ttcagaaggt tatgagacaa gaagacatta gtgagatatg
      241 gtttgaatat gaaggcacac cactgaaatg gcattatcca attggtttgc tatttgatct
      301 tcttgcatca agttcagctc ttccttggaa catcacagta cattttaaga gttttccaga
      361 aaaagacctt ctgcactgtc catctaagga tgcaattgaa gctcatttta tgtcatgtat
      421 gaaagaagct gatgctttaa aacataaaag tcaagtaatc aatgaaatgc agaaaaaaga
      481 tcacaagcaa ctctggatgg gattgcaaaa tgacagattt gaccagtttt gggccatcaa
      541 tcggaaactc atggaatatc ctgcagaaga aaatggattt cgttatatcc cctttagaat
      601 atatcagaca acgactgaaa gacctttcat tcagaagctg tttcgtcctg tggctgcaga
      661 tggacagttg cacacactag gagatctcct caaagaagtt tgtccttctg ctattgatcc
      721 tgaagatggg gaaaaaaaga atcaagtgat gattcatgga attgagccaa tgttggaaac
      781 acctctgcag tggctgagtg aacatctgag ctacccggat aattttcttc atattagtat
      841 catcccacag ccaacagatg ggagtggcag tcgcggacag agtggtggac cggtcgccac
      901 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      961 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1021 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1081 cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     1141 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1201 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1261 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1321 caagctggag tacaactaca tcagcgacaa cgtctatatc accgccgaca agcagaagaa
     1381 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     1441 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1501 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1561 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     1621 aagcggccgc gactctagat cataatcagc catac
//
