LOCUS       sequence-85938-e        1280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1280)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG12-N-18
FEATURES             Location/Qualifiers
     source          1..1280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      514..523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(520..522,523..525,526..1239)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(565..586)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(826..845)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1173..1194
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagg ccaccatggc ggaggagccg
       61 cagtctgtgt tgcagcttcc tacttcaatt gctgctggag gggaaggact tacggatgtc
      121 tccccagaaa caaccacccc ggagcccccg tcttccgctg cagtttcccc gggaacagag
      181 gaacctgctg gcgacaccaa gaaaaaaatt gacattttgc taaaggctgt gggagacact
      241 cctattatga aaacaaagaa gtgggcagta gagcgaacac gaaccatcca aggactcatt
      301 gacttcatca aaaagtttct taaacttgtg gcctcagaac agttgtttat ttatgtgaat
      361 cagtcctttg ctccttcccc agaccaagaa gttggaactc tctatgagtg ttttggcagt
      421 gatggtaaac tggttttaca ttactgcaag tctcaggcgt ggggaggcag cggtggaggc
      481 agcgcatccg gcggaagcgg aagcggaccg gtcgccacca tggtgagcaa gggcgaggag
      541 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      601 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      661 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg
      721 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      781 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      841 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      901 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
      961 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     1021 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     1081 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1141 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1201 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     1261 taatcagcca taccacattg
//
