LOCUS       sequence-85940-e        2411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2411)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG13-N-18
FEATURES             Location/Qualifiers
     source          1..2411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1635..1644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1641..1643,1644..1646,1647..2360)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1686..1707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1947..1966)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2294..2315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acaatgctag tgacgtcaga tccgctagcg ccaccatgga aactgatctc aattcccagg
       61 acagaaagga cctggacaag tttattaaat tttttgccct caagactgtc caagtgattg
      121 tccaggctcg gcttggtgaa aagatttgca ctcgttcatc atcttctcca acgggttcag
      181 attggttcaa cttagcaatc aaagacatcc cagaggttac acatgaagca aagaaggcac
      241 tggcaggaca gctgcctgca gtcgggaggt ccatgtgtgt ggagatttca cttaagactt
      301 ctgagggaga ttccatggag ctggaaatat ggtgtcttga aatgaatgaa aagtgtgata
      361 aagaaatcaa agtttcctac acggtgtaca acagactgtc attgctgctg aagtcccttc
      421 ttgctataac tagggtgaca ccagcctata ggctctccag gaaacaaggg catgaatatg
      481 tcatattata caggatatat tttggagaag ttcagctgag tggcttagga gaaggcttcc
      541 agacagttcg tgttgggaca gtgggcaccc ctgtgggcac catcactctt tcttgtgctt
      601 acagaattaa cttggcattc atgtctacca ggcaatttga gaggacccca cctatcatgg
      661 ggattattat tgatcacttt gtggaccgtc cctatcccag ctcctctccc atgcacccct
      721 gcaattacag aactgctggt gaggacactg gagtaatata cccgtctgta gaagactctc
      781 aagaagtgtg taccacctct ttttccacct ccccaccatc ccagctctca agctctcgcc
      841 tttcctatca gcctgctgcc ctgggcgttg gatcagctga cctggcttat ccagtagtgt
      901 ttgctgctgg cttaaatgct acacaccctc accagctgat ggttcctggg aaggaaggtg
      961 gggtacccct tgctcccaac cagcctgtcc atggtaccca ggctgaccag gagagactgg
     1021 caacctgcac cccttctgac agaacccact gtgctgccac accctccagt agtgaggata
     1081 ctgaaaccgt atcaaacagc agtgagggac gggcctcccc tcacgatgtc ttggagacca
     1141 tctttgtccg aaaagtgggg gcttttgtca acaaacccat taaccaggtg accctgacga
     1201 gtttggatat accctttgcc atgtttgctc ccaagaattt ggagctggag gataccgatc
     1261 caatggtgaa tcctccagat tccccagaga ctgaatctcc tctccagggc agcctgcact
     1321 cagatggctc cagcgggggc agcagtggca atacccatga tgactttgtt atgatagact
     1381 ttaaaccagc tttttctaaa gatgacattc ttccgatgga cctggggacc ttctatcggg
     1441 agtttcagaa cccacctcag ctgagcagcc tctccataga tattggagca cagtccatgg
     1501 ctgaagactt ggactcatta ccagagaagc tggctgtgca tgagaagaat gtccgcgagt
     1561 ttgatgcctt tgtggaaacc ctgcagagca gcggtggagc aagcgcagcc agtggtagcg
     1621 cggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1681 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1741 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1801 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
     1861 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1921 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1981 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2041 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
     2101 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     2161 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2221 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     2281 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2341 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
     2401 gagaagttag c
//
