LOCUS       sequence-85941-e        2349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2349)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG14-C-18
FEATURES             Location/Qualifiers
     source          1..2349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agcgacaacg
      481 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
      541 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc tggcagcggt ggaggcagcg
      781 catccggcgg aagcggaagc gcgtctccca gtgggaaggg agcccgggcg ctggaggctc
      841 ctggctgcgg gccccggccg ctcgcccggg acctggtgga ctccgtggac gatgcggagg
      901 ggctgtacgt ggctgtggag cgctgcccgc tgtgcaacac tacccgccgg cggctgacct
      961 gcgccaaatg cgttcagagc ggcgatttcg tctacttcga cggccgcgac cgggagaggt
     1021 ttatcgacaa gaaggaaagg ttaagccgac ttaagagcaa gcaagaagaa tttcagaaag
     1081 aagtgttaaa agctatggaa ggaaaatgga taacagatca gttgagatgg aaaataatgt
     1141 cctgcaagat gaggattgaa cagttaaaac aaacaatatg taaaggaaat gaagaaatgg
     1201 agaaaaattc tgaaggcctt ctcaaaacca aggaaaagaa tcagaagctt tacagtcgag
     1261 cacaacggca ccaagagaaa aaggagaaga ttcagaggca taatcgcaaa cttggtgacc
     1321 tggtagaaaa aaagaccatt gacttaagaa gtcattatga gcgtctggca aatcttcgac
     1381 gatcccatat attagagctc acctctgtca tttttccaat cgaggaagta aagacgggtg
     1441 tgagagaccc cgcagatgtg tcttcagaga gtgacagtgc catgacctcc agcactgtga
     1501 gcaagcttgc tgaagcccgg aggacaactt acctctcagg acgatgggtc tgtgacgatc
     1561 acaacggaga caccagcatt agcattacag ggccttggat tagcctccct aacaatgggg
     1621 actactctgc ctactacagc tgggtggagg agaagaaaac aacccagggg cctgacatgg
     1681 agcagagtaa ccctgcctac accatcagtg ctgcgctgtg ctatgcaact cagctggtca
     1741 acattctgtc tcatatactt gatgtaaatc ttcccaaaaa gctctgcaac agtgaatttt
     1801 gtggcgaaaa tctaagcaag cagaaattta ctcgagcagt gaagaaactg aatgcaaata
     1861 ttctttacct ttgtttttct cagcatgtaa atttagatca attacaacca ctgcataccc
     1921 tcaggaatct aatgtacctg gtcagtccaa gctctgaaca cctaggcagg tcagggccct
     1981 ttgaagtacg agcagacctt gaggagtcca tggaatttgt ggatcccgga gttgctggag
     2041 aatcagatga gagcggagat gagcgcgtca gcgatgaaga aaccgacctg ggcacagact
     2101 gggagaactt gcctagtccc cggttttgtg atatcccttc ccagtctgtg gaagtctccc
     2161 agagtcagag cacccaggcg tccccaccca tcgcgagcag cagtgcaggt gggatgatct
     2221 cctctgcagc agcctcggtg acctcctggt ttaaagctta cactggacac cgttaagaat
     2281 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     2341 agccatacc
//
