LOCUS       sequence-85943-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG16-C-18
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt cctggggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcga
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      541 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gggagatagt ggtgggagcg gttcatcgtc gggcctgcgc gccgcagact ttccccgctg
      841 gaagcgtcac atcgcggagg aactgaggcg ccgggaccga ctgcagaggc aggcgttcga
      901 ggagatcatt ctgcagtata ccaagttgct ggaaaagtca gatcttcatt cagtattgac
      961 ccagaaacta caagcagaaa agcatgacat gccaaatagg catgaaataa gtcctggaca
     1021 tgatggtgcg tggaatgata gtcaactaca agaaatggcc cagttgagga tcaaacacca
     1081 ggaagagctg accgaactgc acaagaagcg tggggagtta gctcagttgg tgattgacct
     1141 gaacaaccaa atgcagcaga aggacaagga gatacagatg aatgaagcaa agatttcgga
     1201 gtatttacag accatctctg acctggagac aaactgcctg gacctgcgca ccaaactgca
     1261 ggacctcgag gtagccaacc agaccctgaa ggatgagtat gacgccctgc agattacttt
     1321 tactgcccta gaagagaaac tgaggaaaac tactgaggag aaccaggaac tggtcaccag
     1381 atggatggct gagaaggccc aagaagccaa tcgcctcaat gcagagaatg agaaggactc
     1441 caggaggcgt caagcacggc tgcagaagga gcttgcagaa gcagcaaagg aacctctacc
     1501 tgttgaacag gatgatgaca ttgaagtcat tgtggatgag acctcagacc acacagaaga
     1561 gacctctccc gtccgagctg tcagcagagc agctacgaga cgctctgtct cttccatccc
     1621 agtcccccag gatatcatgg acactcatcc tgcttctggt aaagatgtga gagtcccaac
     1681 tactgcctcg tatgtcttcg atgcgcatga cggagaggtc aacgcagtgc agttcagtcc
     1741 aggctcccgg ttgctggcca ctggaggcat ggaccgcagg gtgaaacttt gggaagcatt
     1801 cggagataaa tgtgaattca agggctccct gtctggcagt aatgctggaa ttacaagcat
     1861 tgaatttgat agtgctggag cttacctatt agcagcttca aatgattttg caagccgaat
     1921 ctggactgtg gatgattatc gattacggca cacactcaca ggccacagcg ggaaagtcct
     1981 ctctgccaag ttcctgctgg acaatgcacg gattgtctca ggaagtcacg accggaccct
     2041 caaactctgg gatctccgca gcaaagtctg cataaaaaca gtgtttgcag gatccagctg
     2101 caatgacatt gtttgcactg aacaatgtgt aatgagtgga cattttgaca agaaaattcg
     2161 tttctgggat atccggtcag agagtgtggt ccgagagatg gaactgttag ggaagatcac
     2221 tgctctggac ctaaaccctg agagaactga gctcctgagc tgctcccgtg atgacctgct
     2281 aaaagtcatc gacctccgga caaatgcagt caaacagaca ttcagtgcgc ctggattcaa
     2341 atgcggctct gactggaccc gggttgtctt cagccctgat ggcagttacg tggcagcagg
     2401 ctcagccgag ggttctcttt atgtctggag tgtgctgaca gggaaagtgg agaaggttct
     2461 ttcaaaacag cacagctctt ctatcaatgc ggtggcgtgg gccccctcgg gcttacatgt
     2521 tgtcagtgtg gacaaaggaa gcagagctat gctgtgggca cagccttgag gtaccgcggg
     2581 cccgggatcc accggatcta gataactgat cataatcagc catacca
//
