LOCUS       sequence-85945-e        2411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2411)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..90
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1663..1672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1669..1671,1672..1674,1675..2388)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1714..1735)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1975..1994)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2322..2343
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcat ggggagcagc
       61 aagagcaagc ccaaggaccc cagccagcgc cggcgcagcc tggagccacc cgacagcacc
      121 caccacgggg gattcccagc ctcgcagacc cccaacaaga cagcagcccc cgacacgcac
      181 cgcaccccca gccgctcctt tgggaccgtg gccaccgagc ccaagctctt cgggggcttc
      241 aacacttctg acaccgtcac gtcgccgcag cgtgccgggg cactggctgg cggcgtcacc
      301 actttcgtgg ctctctatga ctacgagtcc cggactgaaa cggacttgtc cttcaagaaa
      361 ggagaacgcc tgcagattgt caacaacacg gaaggtgact ggtggctggc tcattccctc
      421 actacaggac agacgggcta catccccagt aactatgtcg cgccctcaga ctccatccag
      481 gctgaagagt ggtactttgg gaagatcact cgtcgggagt ccgagcggct gctgctcaac
      541 cccgaaaacc cccggggaac cttcttggtc cgggagagcg agacgacaaa aggtgcctat
      601 tgcctctccg tttctgactt tgacaacgcc aaggggctca atgtgaagca ctacaagatc
      661 cgcaagctgg acagcggcgg cttctacatc acctcacgca cacagttcag cagcctgcag
      721 cagctggtgg cctactactc caaacatgct gatggcttgt gccaccgcct gaccaacgtc
      781 tgccccacgt ccaagcccca gacccaggga ctcgccaagg acgcgtggga aatcccccgg
      841 gagtcgctgc ggctggaggt gaagctgggg cagggctgct ttggagaggt ctggatgggg
      901 acctggaacg gcaccaccag agtggccata aagactctga agcccggcac catgtccccg
      961 gaggccttcc tgcaggaagc ccaagtgatg aagaagctcc ggcatgagaa gctggttcag
     1021 ctgtacgcag tggtgtcgga agagcccatc tacatcgtca ctgagtacat gagcaagggg
     1081 agcctcctgg atttcctgaa gggagagatg ggcaagtacc tgcggctgcc acagctcgtc
     1141 gatatggctg ctcagattgc atccggcatg gcctatgtgg agaggatgaa ctacgtgcac
     1201 cgagacctgc gggcggccaa catcctggtg ggggagaacc tggtgtgcaa ggtggctgac
     1261 tttgggctgg cacgcctcat cgaggacaac gagtacacag cacggcaagg tgccaagttc
     1321 cccatcaagt ggacagcccc cgaggcagcc ctctatggcc ggttcaccat caagtcggat
     1381 gtctggtcct tcggcatcct gctgactgag ctgaccacca agggccgggt gccataccca
     1441 gggatggtca acagggaggt gctggaccag gtggagaggg gctaccgcat gccctgcccg
     1501 cccgagtgcc ccgagtcgct gcatgacctc atgtgccagt gctggcggaa ggaccctgag
     1561 gagcggccca cttttgagta cctgcaggcc ttcctggagg actacttcac ctcgacagag
     1621 ccccagtacc agcctggaga gaacctacgg gatccaccgg tcgccaccat ggtgagcaag
     1681 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1741 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1801 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1861 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1921 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1981 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2041 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2101 aactacatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     2161 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     2221 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2281 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2341 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     2401 tctagatcat a
//
