LOCUS       sequence-85947-e        2593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2593)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1845..1854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1851..1853,1854..1856,1857..2570)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1896..1917)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2157..2176)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2504..2525
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagaccg ccatggaagg gaagtggttg ctgtgtatgt
       61 tactggtgct tggaactgct attgttgagg ctcatgatgg acatgatgat gatgtgattg
      121 atattgagga tgaccttgac gatgtcattg aagaggtaga agactcaaaa ccagatacca
      181 ctgctcctcc ttcatctccc aaggttactt acaaagctcc agttccaaca ggggaagtat
      241 attttgctga ttcttttgac agaggaactc tgtcagggtg gattttatcc aaagccaaga
      301 aagacgatac cgatgatgaa attgccaaat atgatggaaa gtgggaggta gaggaaatga
      361 aggagtcaaa gcttccaggt gataaaggac ttgtgttgat gtctcgggcc aagcatcatg
      421 ccatctctgc taaactgaac aagcccttcc tgtttgacac caagcctctc attgttcagt
      481 atgaggttaa tttccaaaat ggaatagaat gtggtggtgc ctatgtgaaa ctgctttcta
      541 aaacaccaga actcaacctg gatcagttcc atgacaagac cccttatacg attatgtttg
      601 gtccagataa atgtggagag gactataaac tgcacttcat cttccgacac aaaaacccca
      661 aaacgggtat ctatgaagaa aaacatgcta agaggccaga tgcagatctg aagacctatt
      721 ttactgataa gaaaacacat ctttacacac taatcttgaa tccagataat agttttgaaa
      781 tactggttga ccaatctgtg gtgaatagtg gaaatctgct caatgacatg actcctcctg
      841 taaatccttc acgtgaaatt gaggacccag aagaccggaa gcccgaggat tgggatgaaa
      901 gaccaaaaat cccagatcca gaagctgtca agccagatga ctgggatgaa gatgcccctg
      961 ctaagattcc agatgaagag gccacaaaac ccgaaggctg gttagatgat gagcctgagt
     1021 acgtacctga tccagacgca gagaaacctg aggattggga tgaagacatg gatggagaat
     1081 gggaggctcc tcagattgcc aaccctagat gtgagtcagc tcctggatgt ggtgtctggc
     1141 agcgacctgt gattgacaac cccaattata aaggcaaatg gaagcctcct atgattgaca
     1201 atcccagtta ccagggaatc tggaaaccca ggaaaatacc aaatccagat ttctttgaag
     1261 atctggaacc tttcagaatg actcctttta gtgctattgg tttggagctg tggtccatga
     1321 cctctgacat tttttttgac aactttatca tttgtgctga tcgaagaata gttgatgatt
     1381 gggccaatga tggatggggc ctgaagaaag ctgctgatgg ggctgctgag ccaggcgttg
     1441 tggggcagat gaacgaggca gctgaagagc gcccgtggct gtgggtagtc tatattctaa
     1501 ctgtagccct tcctgtgttc ctggttatcc tcttctgctg ttctggaaag aaacagacca
     1561 gtggtatgga gtataagaaa actgatgcac ctcaaccgga tgtgaaggaa gaggaagaag
     1621 agaaggaaga ggaaaaggac aagggagatg aggaggagga aggagaagag aaacttgaag
     1681 agaaacagaa aagtgatgct gaagaagatg gtggcactgt cagtcaagag gaggaagaca
     1741 gaaaacctaa agcagaggag gatgaaattt tgaacagatc accaagaaac agaaagccac
     1801 gaagagaggg gggtgcgtca ggcggcagcg gatctggacc ggtcgccacc atggtgagca
     1861 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1921 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1981 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2041 ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2101 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2161 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2221 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2281 acaactacat cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     2341 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     2401 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2461 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2521 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     2581 actctagatc ata
//
