LOCUS       sequence-85949-e        1161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1161)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-CEBPA237-C-4
FEATURES             Location/Qualifiers
     source          1..1161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(17..19,20..22,23..733)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(62..83)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(323..342)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     670..691
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accccggttc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
       61 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      121 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      181 cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta
      241 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      301 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      361 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      421 caacatcctg gggcacaagc tggagtacaa ctacatcagc gacaacgtct atatcaccgc
      481 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
      541 cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      601 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
      661 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      721 cgagctgtac aagtccggac ctgcaatggg tgctgcgggc ctgccgggcc ccgggggctc
      781 gctcaagggc ttggctggtc cgcaccccga cctccgcacc ggcggcggcg gcggcggcgg
      841 ggccggcgcg ggcaaggcca agaagtcggt ggataagaac agcaacgagt accgggtacg
      901 gcgggaacgc aacaacatcg cggtgcgcaa gagccgagat aaagccaaac agcgcaacgt
      961 ggagacgcag cagaaggtgt tggagttgac cagtgacaat gaccgcctgc gcaagcgggt
     1021 ggaacagctg agccgtgaac tggacacgct gcggggtatc ttccgccagc tgcctgagag
     1081 ctccttggtc aaggccatgg gcaactgcgc gtgaggcgcg cggatccacc ggatctagat
     1141 aactgatcat aatcagccat a
//
