LOCUS       sequence-85950-e        1535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: , Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1535)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-CIDEB-C-18
FEATURES             Location/Qualifiers
     source          1..1535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..50,51..53,54..764)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(93..114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(354..373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     701..722
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgccctgcct agtgacgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg
       61 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      121 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      241 tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      301 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      361 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      421 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      481 actacatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      541 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
      601 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      661 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      721 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatcta
      781 gtagcggagg tagcgcatcc ggcggaagcg gaggaagcga gtacctctca gctctgaacc
      841 ccagtgactt actcaggtca gtatctaata taagctcgga gtttggacgg agggtctgga
      901 cctcagctcc accaccccag cgacctttcc gtgtctgtga tcacaagcgg accatccgga
      961 aaggcctgac agctgccacc cgccaggagc tgctagccaa agcattggag accctactgc
     1021 tgaatggagt gctaaccctg gtgctagagg aggatggaac tgcagtggac agtgaggact
     1081 tcttccagct gctggaggat gacacgtgcc tgatggtgtt gcagtctggt cagagctgga
     1141 gccctacaag gagtggagtg ctgtcatatg gcctgggacg ggagaggccc aagcacagca
     1201 aggacatcgc ccgattcacc tttgacgtgt acaagcaaaa ccctcgagac ctctttggca
     1261 gcctgaatgt caaagccaca ttctacgggc tctactctat gagttgtgac tttcaaggac
     1321 ttggcccaaa gaaagtactc agggagctcc ttcgttggac ctccacactg ctgcaaggcc
     1381 tgggccatat gttgctggga atttcctcca cccttcgtca tgcagtggag ggggctgagc
     1441 agtggcagca gaagggccgc ctccattcct actaaggatc caccggatct agataactga
     1501 tcataatcag ccataccaca ttgtagaaaa tagcc
//
