LOCUS       sequence-85951-e        1448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1448)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
      781 acatggctga tgactttggc ttcttctcgt cgtcggagag tggtgccccg gaggcggcgg
      841 aggaggaccc ggcggccgcc ttcctggccc agcaggagag cgagattgca ggcatagaga
      901 acgacgaggg cttcggggca cctgccggca gccatgcggc ccccgcacag ccgggcccca
      961 cgagtggggc tggttctgag gacatgggga ccacagtcaa tggagatgtg tttcaggagg
     1021 ccaacggtcc tgctgatggc tacgcagcca ttgcccaggc tgacaggctg acccaggagc
     1081 ctgagagcat ccgcaagtgg cgagaggagc agaggaaacg gctgcaagag ctggatgctg
     1141 catctaaggt cacggaacag gaatggcggg agaaggccaa gaaggacctg gaggagtgga
     1201 accagcgcca gagtgaacaa gtagagaaga acaagatcaa caaccgggca tccgaggagg
     1261 ctttcgtgaa ggaatccaag gaggagaccc caggcacaga gtgggagaag gtggcccagc
     1321 tatgtgactt caaccccaag agcagcaagc agtgcaaaga tgtgtcccgc ctgcgctcgg
     1381 tgctcatgtc cctgaagcag acgccactgt cccgctaggg atccaccgga tctagataac
     1441 tgatcata
//
