LOCUS       sequence-85952-e        1548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1548)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(790..792,793..795,796..1509)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(835..856)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1096..1115)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1443..1464
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagctca agcttcgaat tcgcggctgc acgctcctcg
       61 ggacacagtg ccaaccatcc agaggacaag atggattggg gcacactaca gagcatcctc
      121 gggggtgtca acaagcactc caccagcatt gggaaaatct ggctcactgt cctcttcatc
      181 ttccgcatca tgatcctcgt ggtggccgcg aaggaggtgt ggggagatga gcaagccgat
      241 tttgtttgca acactctcca gcctggctgt aagaatgtgt gctacgacca ctacttcccc
      301 atctctcaca tccggctctg ggctctgcag ctgatcatgg tgtccacgcc ggccctcctg
      361 gtagctatgc acgtggccta ccggagacac gaaaagaaac ggaagttcat gaagggagag
      421 ataaagaacg agtttaagga catcgaagag atcaaaaccc agaaggtccg tatcgaaggg
      481 tccctgtggt ggacctacac caccagcatc ttcttccggg tcatcttcga agctgtcttc
      541 atgtatgtct tttacatcat gtacaatggc ttcttcatgc agcgtctggt gaagtgtaac
      601 gcctggcctt gtcccaatac agtggactgc ttcatttcca ggcccacaga aaagactgtc
      661 ttcacggtgt tcatgatctc tgtgtctgga atttgcatcc tgctaaacat cacagagctg
      721 tgctatctgt tcattaggta ttgctcaggg aagtccaaaa gaccagtcgc ggatccaccg
      781 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      901 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      961 tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac
     1021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1081 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1201 ctggggcaca agctggagta caactacatc agcgacaacg tctatatcac cgccgacaag
     1261 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     1321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1381 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1501 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacat
//
