LOCUS       sequence-85956-e        1455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1455)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Endo-14
FEATURES             Location/Qualifiers
     source          1..1455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             783..812
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggatcagatc tcgagctcaa gcttgatatc gaattcccca
      781 tggagcagaa gctgatctcc gaggaggacc tgaaccggaa gaaactggtg gttgttggtg
      841 atggagcctg tggaaagaca tgcttgctca tagtcttcag caaggacgag ttcccagagg
      901 tgtatgtgcc cacagtgttt gagaactatg tggcagatat cgaggtggat ggaaagcagg
      961 tagagttggc tttgtgggac acagctggcc aggaggacta cgaccgcctg cggccgctct
     1021 cctacccgga caccgacgtc attctcatgt gcttctcggt ggacagcccg gactcgctgg
     1081 agaacatccc cgagaagtgg gtccccgagg tgaagcactt ctgtcccaat gtgcccatca
     1141 tcctggtggc caacaaaaaa gacctgcgca gcgacgagca tgtccgcaca gagctggccc
     1201 gcatgaagca ggaacccgtt cgcacggatg acggccgcgc catggccgtg cgcatccaag
     1261 cctacgacta cctcgagtgc tctgccaaga ccaaggaagg cgtgcgcgag gtcttcgaga
     1321 cggccacgcg cgccgcgctg cagaagcgct acggctccca gaacggctgc atcaactgct
     1381 gcaaggtgct atgagggccg cgcccgtcgc gcctgcccct gccggggatc caccggatct
     1441 agataactga tcata
//
