LOCUS       sequence-85959-e        1730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 434, Emission: 434.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1730)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1533..1544)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggactcagat ctcgagccat gaagccagga ttcagtcccc
      781 gtgggggtgg ctttggcggc cgagggggct ttggtgaccg tggtggtcgt ggaggccgag
      841 ggggctttgg cgggggccga ggccaaggcg gaggctttag aggtcgtgga cgaggaggag
      901 gtggaggcgg cggcggcggt ggaggaggag gaagaggtgg tggaggcttc cattttggtg
      961 gcaaccgggg tcgtggtcgg ggaggaaaaa gaggaaacca gtcggggaag aatgtgatgg
     1021 tggagccgca tcggcatgag ggtgtcttca tttgtcgggg aaaggaagat gcactggtca
     1081 ccaagaacct ggtccctggg gaatcagttt atggagagaa gagagtctcg atttcggaag
     1141 gagatgacaa aattgagtac cgagcctgga accccttccg ctccaagctt gcagcagcaa
     1201 tcctgggtgg tgtggaccag atccacatca aaccgggggc taaggttctc tacctcgggg
     1261 ctgcctcggg caccacggtc tcccatgtct ctgacatcgt tggtccggat ggtctagtct
     1321 atgcagtcga gttctcccac cgctctggcc gtgacctcat taacttggcc aagaagagga
     1381 ccaacatcat tcctgtgatc gaggatgctc gacacccaca caaataccgc atgctcatcg
     1441 caatggtgga tgtgatcttt gctgatgtgg cccagccaga ccagacccgg attgtggccc
     1501 tgaatgccca caccttcctg cgtaatggag gacactttgt gatttccatt aaggccaact
     1561 gcattgactc cacagcctca gccgaggccg tgtttgcctc cgaagtgaaa aagatgcaac
     1621 aggagaacat gaagccgcag gagcagttga cccttgagcc atatgaaaga gaccatgccg
     1681 tggtcgtggg agtgtacagg ccacccccca aggtgaagaa ctgaggatcc
//
