LOCUS       sequence-85961-e        2411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 434, Emission:
            434.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2411)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-FYN2-N-10
FEATURES             Location/Qualifiers
     source          1..2411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1660..1669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1666..1668,1669..1671,1672..2385)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1711..1732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1972..1991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2319..2340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgggc tgtgtgcaat gtaaggataa agaagcaaca
       61 aaactgacgg aggagaggga cggcagcctg aaccagagct ctgggtaccg ctatggcaca
      121 gaccccaccc ctcagcacta ccccagcttc ggtgtgacct ccatccccaa ctacaacaac
      181 ttccacgcag ccgggggcca aggactcacc gtctttggag gtgtgaactc ttcgtctcat
      241 acggggacct tgcgtacgag aggaggaaca ggagtgacac tctttgtggc cctttatgac
      301 tatgaagcac ggacagaaga tgacctgagt tttcacaaag gagaaaaatt tcaaatattg
      361 aacagctcgg aaggagattg gtgggaagcc cgctccttga caactggaga gacaggttac
      421 attcccagca attatgtggc tccagttgac tctatccagg cagaagagtg gtactttgga
      481 aaacttggcc gaaaagatgc tgagcgacag ctattgtcct ttggaaaccc aagaggtacc
      541 tttcttatcc gcgagagtga aaccaccaaa ggtgcctatt cactttctat ccgtgattgg
      601 gatgatatga aaggagacca tgtcaaacat tataaaattc gcaaacttga caatggtgga
      661 tactacatta ccacccgggc ccagtttgaa acacttcagc agcttgtaca acattactca
      721 gagagagctg caggtctctg ctgccgccta gtagttccct gtcacaaagg gatgccaagg
      781 cttaccgatc tgtctgtcaa aaccaaagat gtctgggaaa tccctcgaga atccctgcag
      841 ttgatcaaga gactgggaaa tgggcagttt ggggaagtat ggatgggtac ctggaatgga
      901 aacacaaaag tagccataaa gactcttaaa ccaggcacaa tgtcccccga atcattcctt
      961 gaggaagcgc agatcatgaa gaagctgaag cacgacaagc tggtccagct ctatgcagtg
     1021 gtgtctgagg agcccatcta catcgtcacc gagtatatga acaaaggaag tttactggat
     1081 ttcttaaaag atggagaagg aagagctctg aaattaccaa atcttgtgga catggcagca
     1141 caggtggctg caggaatggc ttacatcgag cgcatgaatt atatccatag agatctgcga
     1201 tcagcaaaca ttctagtggg gaatggactc atatgcaaga ttgctgactt cggattggcc
     1261 cgattgatag aagacaatga gtacacagca agacaaggtg caaagttccc catcaagtgg
     1321 acggcccccg aggcagccct gtacgggagg ttcacaatca agtctgacgt gtggtctttt
     1381 ggaatcttac tcacagagct ggtcaccaaa ggaagagtgc catacccagg catgaacaac
     1441 cgggaggtgc tggagcaggt ggagcgaggc tacaggatgc cctgcccgca ggactgcccc
     1501 atctctctgc atgagctcat gatccactgc tggaaaaagg accctgaaga acgccccact
     1561 tttgagtact tgcagagctt cctggaagac tactttaccg cgacagagcc ccagtaccaa
     1621 cctggtgaaa acctggcatc cagcggggat ccaccggtcg ccaccatggt gagcaagggc
     1681 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1741 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1801 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1861 tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1921 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1981 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2041 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2101 tacatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     2161 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     2221 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2281 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2341 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     2401 agatcataat c
//
