LOCUS       sequence-85963-e        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-H2A-10
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      435..444
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(441..443,444..446,447..1160)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(486..507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(747..766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1094..1115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agccgccacc atgtcgggac gtggcaagca gggcggcaaa gctcgcgcca
       61 aggccaagac ccgctcttct cgggccgggc ttcagtttcc cgtaggccga gtgcatcgcc
      121 tgctccgcaa aggcaactat gcggagcggg tcggtgctgg agcgccggtg tacctggcgg
      181 cggtgctgga gtacctgacc gccgagatcc tggagctggc tggcaacgcg gcccgcgaca
      241 acaagaagac tcgcatcatc ccgcgtcacc tccagctggc catccgcaac gatgaggagc
      301 tcaacaagct tctgggcaaa gtcaccatcg cacagggtgg cgtcctgccc aacatccagg
      361 ccgtgctact gcccaagaag accgagagcc accacaaggc gaagggcaag tcgggaagca
      421 cggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      481 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      541 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      601 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      661 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      721 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      781 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      841 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      901 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      961 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1021 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     1081 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1141 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
//
