LOCUS       sequence-85966-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-H4-23
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    335..387
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(401..403,404..406,407..1120)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(446..467)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(707..726)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1054..1075
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagccgcca ccatgtctgg ccgcggcaaa ggcgggaagg gtcttggcaa
       61 aggcggcgct aagcgccacc gtaaagtact gcgcgacaat atccagggca tcaccaagcc
      121 ggccatccgg cgccttgctc gccgcggcgg cgtgaagcgc atctccggcc tcatctacga
      181 ggagactcgc ggggtgctga aggtgttcct ggagaacgtg atccgggacg ccgtgaccta
      241 tacagagcac gccaagcgca agacggtcac cgccatggat gtggtctacg cgctcaagcg
      301 ccagggccgc accctctacg gtttcggtgg ggatctcgag ctcaagcttc gaattctgca
      361 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
      421 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      481 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      541 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg
      601 gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      661 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      721 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      781 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat
      841 cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
      901 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
      961 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1021 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1081 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
     1141 ataatcagc
//
