LOCUS       sequence-85972-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(22..24,25..27,28..738)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(67..88)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(328..347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     675..696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca tcagcgacaa cgtctatatc
      481 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      541 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgat ggcgactgcg
      781 acccccgtgc cgccgcggat gggcagccgc gctggcggcc ccaccacgcc gctgagcccc
      841 acgcgcctgt cgcggctcca ggagaaggag gagctgcgcg agctcaatga ccggctggcg
      901 gtgtacatcg acaaggtgcg cagcctggag acggagaaca gcgcgctgca gctgcaggtg
      961 acggagcgcg aggaggtgcg cggccgtgag ctcaccggcc tcaaggcgct ctacgagacc
     1021 gagctggccg acgcgcgacg cgcgctcgac gacacggccc gcgagcgcgc caagctgcag
     1081 atcgagctgg gcaagtgcaa ggcggaacac gaccagctgc tcctcaacta tgctaagaag
     1141 gaatctgatc ttaatggcgc ccagatcaag cttcgagaat atgaagcagc actcaattcg
     1201 aaagatgcag ctcttgctac tgcacttggt gacaaaaaaa gtttagaggg agatttggag
     1261 gatctgaagg atcagattgc ccagttggaa gcctccttag ctgcagccaa aaaacagtta
     1321 gcagatgaaa ctttacttaa agtagatttg gagaatcgtt gtcagagcct tactgaggac
     1381 ttggagtttc gcaaaagcat gtatgaagag gagattaacg agaccagaag gaagcatgaa
     1441 acgcgcttgg tagaggtgga ttctgggcgt caaattgagt atgagtacaa gctggcgcaa
     1501 gcccttcatg agatgagaga gcaacatgat gcccaagtga ggctgtataa ggaggagctg
     1561 gagcagactt accatgccaa acttgagaat gccagactgt catcagagat gaatacttct
     1621 actgtcaaca gtgccaggga agaactgatg gaaagccgca tgagaattga gagcctttca
     1681 tcccagcttt ctaatctaca gaaagagtct agagcatgtt tggaaaggat tcaagaatta
     1741 gaggacttgc ttgctaaaga aaaagacaac tctcgtcgca tgctgacaga caaagagaga
     1801 gagatggcgg aaataaggga tcaaatgcag caacagctga atgactatga acagcttctt
     1861 gatgtaaagt tagccctgga catggaaatc agtgcttaca ggaaactctt agaaggcgaa
     1921 gaagagaggt tgaagctgtc tccaagccct tcttcccgtg tgacagtatc ccgagcatcc
     1981 tcaagtcgta gtgtacgtac aactagagga aagcggaaga gggttgatgt ggaagaatca
     2041 gaggcgagta gtagtgttag catctctcat tccgcctcag ccactggaaa tgtttgcatc
     2101 gaagaaattg atgttgatgg gaaatttatc cgcttgaaga acacttctga acaggatcaa
     2161 ccaatgggag gctgggagat gatcagaaaa attggagaca catcagtcag ttataaatat
     2221 acctcaagat atgtgctgaa ggcaggccag actgttacaa tttgggctgc aaacgctggt
     2281 gtcacagcca gccccccaac tgacctcatc tggaagaacc agaactcgtg gggcactggc
     2341 gaagatgtga aggttatatt gaaaaattct cagggagagg aggttgctca aagaagtaca
     2401 gtctttaaaa caaccatacc tgaagaagag gaggaggagg aagaagcagc tggagtggtt
     2461 gttgaggaag aacttttcca ccagcaggga accccaagag catccaatag aagctgtgca
     2521 attatgtagg gatccaccgg atctagataa ctgatc
//
