LOCUS       sequence-85973-e        1316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Myosin Light Chain, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1316)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-LC-Myosin-C-10
FEATURES             Location/Qualifiers
     source          1..1316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(22..24,25..27,28..738)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(67..88)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(328..347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     675..696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca tcagcgacaa cgtctatatc
      481 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      541 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgat gtcgagcaag
      781 agagccaagg ccaagaccac caagaagagg ccccagaggg ctacgtccaa tgtcttcgcc
      841 atgtttgacc agtcccagat ccaggagttt aaggaggcct tcaacatgat tgatcagaac
      901 cgcgatggct tcattgataa ggaggacctg cacgacatgt tggcctctct ggggaagaac
      961 cccacagacg agtacctgga gggcatgatg aacgaggcgc cagggcccat caacttcacc
     1021 atgttcctca caatgtttgg ggagaagctg aacggcacag accccgagga tgtgatccgc
     1081 aatgcctttg cctgctttga tgaggaggcc tcaggcttca tccacgagga ccacctgagg
     1141 gagctgctca ccaccatggg cgaccgattc acggatgagg aggtggacga gatgtaccgc
     1201 gaggcaccca ttgataagaa gggcaacttc aactacgtgg agttcactcg catcctcaaa
     1261 cacggcgcca aggacaagga cgactaagat ccaccggatc tagataactg atcata
//
