LOCUS       sequence-85974-e        1580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1580)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-LASP1-C-10
FEATURES             Location/Qualifiers
     source          1..1580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      952..961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggactcagat ctagcggtgg cagtggaaac cccaactgcg
      781 cccggtgcgg caagatcgtg tatcccacgg agaaggtgaa ctgtctggat aagttctggc
      841 ataaagcatg cttccattgc gagacctgca agatgacact gaacatgaag aactacaagg
      901 gctacgagaa gaagccctac tgcaacgcac actaccccaa gcagtccttc accatggtgg
      961 cggacacccc ggaaaacctt cgcctcaagc aacagagtga gctccagagt caggtgcgct
     1021 acaaggagga gtttgagaag aacaagggca aaggtttcag cgtagtggca gacacgcccg
     1081 agctccagag aatcaagaag acccaggacc agatcagtaa cataaaatac catgaggagt
     1141 ttgagaagag ccgcatgggc cctagcgggg gcgagggcat ggagccagag cgtcgggatt
     1201 cacaggacgg cagcagctac cggcggcccc tggagcagca gcagcctcac cacatcccga
     1261 ccagtgcccc ggtttaccag cagccccagc agcagccggt ggcccagtcc tatggtggct
     1321 acaaggagcc tgcagcccca gtctccatac agcgcagcgc cccaggtggt ggcgggaagc
     1381 ggtaccgcgt ggtgtatgac tacagcgccg ccgacgagga cgaggtctcc ttccaggacg
     1441 gggacaccat cgtcaacgtg cagcagatcg acgacggctg gatgtacggg acggtggagc
     1501 gcaccggcga cacggggatg ctgccggcca actacgtgga ggccatctga ggatccaccg
     1561 gatctagata actgatcata
//
