LOCUS       sequence-85975-e        2078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2078)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(40..102,103..1260)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1315..1324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1321..1323,1324..1326,1327..2040)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1366..1387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1627..1646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1974..1995
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agcgccacca tggcggcccc gggcgcccgg
       61 cgtccgctgc tcctgttgct gctggcaggc cttgcacaca gcgccccagc actgttcgag
      121 gtgaaagaca acaacggcac agcgtgtata atggccagct tctctgcctc ctttctgacc
      181 acctatgagg ctggacatgt ttctaaggtc tcgaatatga ccctgccagc ctctgcagaa
      241 gtcctgaaga atagcagctc ttgtggtgaa aagaatgctt ctgagcccac cctcgcaatc
      301 acctttggag aaggatattt actgaaactc accttcacaa aaaacacaac acgttacagt
      361 gtccagcaca tgtatttcac atataacctg tcagacacac aattctttcc caatgccagc
      421 tccaaagggc ccgacactgt ggattccaca actgacatca aggcagacat caacaaaaca
      481 taccgatgtg tcagcgacat cagggtctac atgaagaatg tgaccattgt gctctgggac
      541 gctactatcc aggcctacct gccgagtagc aacttcagca aggaagagac acgctgccca
      601 caggatcaac cttccccaac tactgggcca cccagcccct caccaccact tgtgcccaca
      661 aaccccagtg tgtccaagta caatgtgact ggtgacaatg gaacctgcct gctggcctct
      721 atggcactgc aactcaacat cacctacatg aagaaggaca acacgactgt gaccagagca
      781 ttcaacatca acccaagtga caaatatagt gggacttgcg gtgcccagtt ggtgaccctg
      841 aaggtgggga acaagagcag agtcctggag ctgcagtttg ggatgaatgc cacttctagc
      901 ctgtttttcc tgcaaggagt tcagttgaac atgactcttc ctgatgccat agagcccacg
      961 ttcagcacct ccaactattc cctgaaagct cttcaggcca gtgtcggcaa ctcatacaag
     1021 tgcaacagtg aggagcacat ctttgtcagc aaggcgctcg ccctcaatgt cttcagcgtg
     1081 caagtccagg ctttcagggt agaaagtgac aggtttgggt ctgtggaaga gtgtgtacag
     1141 gacggtaaca acatgctgat ccccattgct gtgggcgggg ccctggcagg gctggtcctc
     1201 atcgtcctca tcgcctacct catcggcagg aagaggagtc acgcgggcta tcagaccatc
     1261 tcggaattcg gctccaccgg ctccaccggc tccaccggcg cggatccacc ggtcgccacc
     1321 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1381 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1441 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1501 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     1561 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1621 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1681 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1741 aagctggagt acaactacat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
     1801 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     1861 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1921 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1981 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     2041 agcggccgcg actctagatc ataatcagcc ataccaca
//
