LOCUS       sequence-85979-e        3814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Membrane , Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3814)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Nesprin3-C-18
FEATURES             Location/Qualifiers
     source          1..3814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(46..48,49..51,52..762)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(91..112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(352..371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     699..720
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      241 tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 tacatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
      541 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      661 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      721 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga
      781 gagtccagtg gatattcgtc tcggtcgggt ggaagcactc agcagcccca ggacgacttt
      841 gacaggagcg tggaggatgc ccaggcatgg atgaaggctg tgcaggacca gctgcaggtc
      901 aatgacaaca cgcagggacc ccgcgcggcc ctggaggcca ggctgtggga gaccgagaaa
      961 atatgccagc tggagcccga ggggcgtgtg agggtggacc tcgtgctacg gatggctgaa
     1021 gccctcttgg catgctgccc tggggaccag aagcccggga tcctggcccg gctgaaggac
     1081 atcaaggccc aatgggagga gacagtcacc tacatgactc actgtcacag ccgcatcgag
     1141 tgggtgtggc tgcactggag cgagtacctg ctggcccgag atgagttcta ccgctggttc
     1201 cagaagatga tggtcacact ggagccccac atcgagctcc agctgggcct gaaggagaag
     1261 cagtggcagc tgagccacgc ccaggtgctg ctgcacaacg tggacaacca ggcggtgctc
     1321 ctggaccggc tgctggagga ggcagcctcc ctgttcaaca ggatcgggga ccccagcgtg
     1381 gacgaagatg cccagaagag aatgaaggct gagtacgatg cagtgaaggc caaggcccag
     1441 aagcgtgtag atctgctgga gcaggtggcc cgggagcatg aggagtacca ggcaggtgtg
     1501 gacgagttcc aactgtggct gaaggcggtg gtggagaagg tgaatggctg cctggggcgg
     1561 aactgcaagc tgcccatcac gcagcgcctc tccacactgc aggacattgc caaagatttt
     1621 cccaggggcg aggagtctct ggagacgctg gaggagcagt ctgcgggtgt cattcggaac
     1681 acctctcctt tgggtgcaga gaagatcacc ggagaactgg aagagatgag gaaagttctg
     1741 gagaagctgc gcgccctctg ggaggaggag gaggagcggc tgcggggcct gctccggtcc
     1801 aggggagcct gggagcagca gattaagcag ctggaggctg agctcagtga gttcaggatg
     1861 gtcctgcaga ggctggccca ggagggcctg cagcctgcgg cgaaagcggg gaccgaggac
     1921 gagctggtgg cacactggag acgctactcg gcaacacggg cggcgctggc ctcggaggag
     1981 ccccgggtgg accggctgca agcccagctg aaggagctca tcgtcttccc tcacaacctg
     2041 aagccactct ctgatagtgt catcgctacc atccaggagt atcagagcct gaaggtgaag
     2101 agcgcgaggc tgcgcaatgc cgcggcggtg gagctgtggc agcatttcca gcggcctctg
     2161 caggatctgc agctgtggaa ggccctggcc cagcggctct tggaggtcac tgccagcctg
     2221 ccggacctgc cttcccttca caccttcctg ccccagatcg aggcagccct gatggaaagc
     2281 tcccgcctga aagagctgct gacgatgctg cagctgaaga aagacctcct gattggcatc
     2341 tttggccagg agagagccac ggcactcctg gagcaggtgg caggttccat gagggacaga
     2401 gacctgctgc ataacagcct cctgcagagg aaaagcaaac tgcagagcct gctcgctcag
     2461 cacaaagact ttggagcagc ttttgagccc ctgcagagga agctcttgga cctccaggtc
     2521 agggtccaag ccgagaaggg gcttcagcgg gaccttcctg gaaaacaggc ccagctctca
     2581 aggttgcagg ggctgcagga agaggggctg gacttggggg cacagatgga ggctgcaagg
     2641 cctctggtcc aggagaaccc caaccaccag cacaaaatgg accagctttc ctccgacttc
     2701 caggccctgc agaggtctct ggaggacctt gtggacaggt gtcggcagag tgtgcaggag
     2761 cactgcacct tcagccacca gctgctggag ctgcggcagt ggatcgttgt gaccacgcaa
     2821 aagctggagg cacaccgggg agaggcgggc ccgggggatg ccgagtccca agaggccgag
     2881 tttgagaggc tggtggcaga attcccggag aaggaggccc agctgtccct ggtggaagcg
     2941 cagggctggc tggtgatgga gaagtcttct ccggagggtg ctgccgtggt gcaggaggag
     3001 ctcagggagc tggcagagtc gtggcgggcc ttgaggctgc tggaagaaag tctgctgagc
     3061 ctcatcagaa actggcatct gcagaggatg gaagtggatt cggggaagaa aatggttttc
     3121 accaacaaca tcccaaagtc aggatttctc atcaatccca tggatcctat tcccaggcat
     3181 cgtcgacgcg caaatcttct acaggaagaa gaagggagcc atgaagattt ctcccagctc
     3241 ctgaggaact ttgggcagtg gttgcaggtg gaaaactcca agctggttag aatcatcgca
     3301 atgagaactt ctacagctga ggacttgagg accagaaaat cgaagctgca ggagctggag
     3361 gctcgggtgc cagaaggtca gcatctcttt gagaacctcc ttcgtctcgg gccagcaagg
     3421 gggacctcgg atgagctgga agatctgcgc taccagtgga tgctgtacaa gtccaagctg
     3481 aaggactctg gccacctgct gacacaaagt tctccagggg agccgactgg attccaaaag
     3541 actcggcggt ggcgaggact gggctccctc ttccggaggg tgtgctgtgt ggcgctccca
     3601 ctgcagctgc ttctgctgct gttcctcctc ctgctgttcc tgctcccaat cagggaagag
     3661 gaccgcagct gcaccctggc caacaacttc gcccgctcct tcacgctcat gctgcgctac
     3721 aatggcccac cacccaccta aggtaccgcg ggcccgggat ccaccggatc tagataactg
     3781 atcataatca gccataccac atgtagaagt ttcc
//
