LOCUS       sequence-85980-e        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1685..1737
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1745..1754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1751..1753,1754..1756,1757..2470)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1796..1817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2057..2076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2404..2425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcaccatg gacgacctcg atgccttact ggcagacctg gaatccacca cctcgcatat
       61 ctctaagcga ccggtgtttc taacagagga aacgccttac tcctacccaa ctggaaacca
      121 tacgtaccag gagattgctg tgccaccgcc ggtgcccccg ccgccttcca gtgaggccct
      181 aaatggcact gtgattgacc ccttagacca gtggcaaccc agcgtgtcca gatacggcca
      241 ccagcaacct cagtcccagt ctccgatata cagctctagt gccaaaagct ccagtgcctc
      301 tgttcctaga gacgggctca gctccccttc tccccgtgcc agtgaggagg aacacgtgta
      361 cagcttccca aacaagcaga agtctgcaga accatctccc acaatgacca gcacctccct
      421 gggcagcaac ctctcggaac tggacaggct tctcctggaa ctgaatgctg ttcaacataa
      481 tccccccagt ggcttctcag cagatgaagt cagcagaagc ccatcactgc ccaatgtgac
      541 cggacctcac tatgtcatcc cagagagcag cagctctgca ggagggaagg ctgcaccccc
      601 tacaaaagaa aaaccaaagc ggaatggtgg gcgtgggatt gaagatgtgc gtcccagtgt
      661 ggagagcctg ctggatgagc tggagagctc tgtgccaagc ccagtccctg caatcactgt
      721 gagccaaggg gaggtgagca gcccccagcg tgtcaacgcc agtcagcagc agacccgtat
      781 ctctgcttct tcagctacac gagaactgga tgagctgatg gcgtccctct ctgactttaa
      841 gttcatggca caggggaaag ccggcggcag cagctcccca ccatccacca ccccaaagcc
      901 tggcagtcag ctggacacca tgctgggaag tctgcagtct gacctgaaca aactgggggt
      961 agcgacggtc gccaagggtg tgtgcggagc ctgcaagaag ccgattgctg ggcaggtagt
     1021 tacagccatg gggaagacct ggcaccctga gcacttcgtc tgcacccact gccaggagga
     1081 gattggatca cggaacttct ttgagcggga tgggcagccc tactgtgaga aggactatca
     1141 caacctcttc tctcctcgct gctactactg caatgggcca atccttgata aagtggtgac
     1201 ggctttggac aggacgtggc accctgaaca ctttttctgt gcccagtgtg gagttttctt
     1261 tggacctgaa ggatttcatg agaaggatgg caaagcctat tgccgcaagg actactttga
     1321 catgtttgct cccaagtgtg gaggctgtgc ccgggctatc ctggaaaatt acatttctgc
     1381 cttaaacacc ctgtggcacc ctgagtgctt tgtctgtcgg gaatgtttca caccgttcat
     1441 caacggcagc ttcttcgagc acgacggcca gccctactgc gaggtgcatt accacgagcg
     1501 ccgcggctcg ctgtgctctg gctgccagaa gcccatcacg ggacgctgca tcactgccat
     1561 gggcaagaaa tttcaccccg aacactttgt ctgtgccttc tgcctcaagc agctcaacaa
     1621 aggaaccttc aaagaacaga acgacaagcc ctactgtcag aactgctttc tcaaactctt
     1681 ctgtctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     1741 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1801 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1861 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1921 ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga
     1981 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2041 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2101 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2161 cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca ccgccgacaa
     2221 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     2281 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2341 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     2401 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2461 gtacaagtaa agcggccgcg actct
//
