LOCUS       sequence-85981-e        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1221..1230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1227..1229,1230..1232,1233..1946)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1272..1293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1533..1552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1880..1901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcacc gccaccatga ggaagatgct cgccgccgtc tcccgcgtgc
       61 tgtctggcgc ttctcagaag ccggcaagca gagtgctggt agcatcccgt aattttgcaa
      121 atgatgctac atttgaaatt aagaaatgtg accttcaccg gctggaagaa ggccctcctg
      181 tcacaacagt gctcaccagg gaggatgggc tcaaatacta caggatgatg cagactgtac
      241 gccgaatgga gttgaaagca gatcagctgt ataaacagaa aattattcgt ggtttctgtc
      301 acttgtgtga tggtcaggaa gcttgctgtg tgggcctgga ggccggcatc aaccccacag
      361 accatctcat cacagcctac cgggctcacg gctttacttt cacccggggc ctttccgtcc
      421 gagaaattct cgcagagctt acaggacgaa aaggaggttg tgctaaaggg aaaggaggat
      481 cgatgcacat gtatgccaag aacttctacg ggggcaatgg catcgtggga gcgcaggtgc
      541 ccctgggcgc tgggattgct ctagcctgta agtataatgg aaaagatgag gtctgcctga
      601 ctttatatgg cgatggtgct gctaaccagg gccagatatt cgaagcttac aacatggcag
      661 ctttgtggaa attaccttgt attttcatct gtgagaataa tcgctatgga atgggaacgt
      721 ctgttgagag agcggcagcc agcactgatt actacaagag aggcgatttc attcctgggc
      781 tgagagtgga tggaatggat atcctgtgcg tccgagaggc aacaaggttt gctgctgcct
      841 attgtagatc tgggaagggg cccatcctga tggagctgca gacttaccgt taccacggac
      901 acagtatgag tgaccctgga gtcagttacc gtacacgaga agaaattcag gaagtaagaa
      961 gtaagagtga ccctattatg cttctcaagg acaggatggt gaacagcaat cttgccagtg
     1021 tggaagaact aaaggaaatt gatgtggaag tgaggaagga gattgaggat gctgcccagt
     1081 ttgccacggc cgatcctgag ccacctttgg aagagctggg ctaccacatc tactccagcg
     1141 acccaccttt tgaagttcgt ggtgccaatc agtggatcaa gtttaagtca gtcagtagct
     1201 ccggagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1261 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1321 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1381 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct
     1441 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc gacaacgtct
     1681 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1741 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1801 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     1861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1921 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     1981 cacat
//
