LOCUS       sequence-85983-e        1393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomal Membrane, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1393)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      638..647
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(644..646,647..649,650..1363)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(689..710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(950..969)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1297..1318
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggactcagat ctgccaccat ggcgccggcc gcgtccaggc tgcgggccga
       61 agccgggctc ggggcgctgc cgcggcgggc gctcgcccag tacttgctct tcctgcggct
      121 ctacccggtg ctcaccaagg cggccaccag tggcattttg tcagcacttg ggaacttcct
      181 ggcccagatg attgagaaga agcggaaaaa agaaaactct agaagtctgg atgtcggtgg
      241 gcctctgaga tatgccgttt acgggttctt cttcacaggg ccgctgagtc acttcttcta
      301 cttcttcatg gaacattgga tccctcctga ggtccccctg gcagggctca ggaggcttct
      361 cctggaccgc ctcgtctttg caccggcctt cctcatgttg ttcttcctca tcatgaactt
      421 tctggagggg aaagacgcct cagccttcgc cgccaagatg agggggggct tctggccggc
      481 gctgaggatg aactggcggg tgtggacgcc actacagttc atcaacatca actacgtccc
      541 tctgaagttc cgggtgctct tcgccaacct ggcagctctg ttctggtatg cctacctggc
      601 ctccttgggg aagagcggct ccggtagcgg accggtcgcc accatggtga gcaagggcga
      661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      781 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc
      841 ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      901 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      961 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1021 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1081 catcagcgac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1141 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     1201 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1261 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1321 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     1381 atcataatca gcc
//
