LOCUS       sequence-85985-e        1437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 
            474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1437)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(22..24,25..27,28..738)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(67..88)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(328..347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     675..696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca tcagcgacaa cgtctatatc
      481 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      541 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaagtc cggactcaga tctcgagcca tggctagtcg aggcgcaaca
      781 agacccaacg ggccaaatac tggaaataaa atatgccagt tcaaactagt acttctggga
      841 gagtctgctg ttggcaaatc aagcctagtg cttcgttttg tgaagggcca atttcatgaa
      901 tttcaagaga gtaccatagg ggctgctttt ctaacccaaa ctgtgtgtct tgatgataca
      961 acagtaaagt ttgaaatatg ggatacagct ggtcaagaac gataccatag cttagcacca
     1021 atgtactaca gaggagcaca agcagccata gttgtatatg atatcacaaa tgaggagtcc
     1081 tttgccagag ccaaaaactg ggttaaagaa cttcagaggc aagccagtcc taacattgta
     1141 atagctttat caggaaacaa ggctgatctt gcaaataaaa gagctgtcga tttccaggaa
     1201 gcacagtcct atgcagatga caacagttta ttattcatgg agacatcagc taaaacatcg
     1261 atgaatgtaa atgaaatatt catggcaata gctaaaaagt tgccaaagaa cgaaccacag
     1321 aatccaggag caaattctgc cagaggaaga ggagtagacc ttactgaacc cacgcagcca
     1381 accaggaatc agtgttgtag taactaagga tccaccggat ctagataact gatcata
//
