LOCUS       sequence-85987-e        1942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1942)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Sequestosome1-C-18
FEATURES             Location/Qualifiers
     source          1..1942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagcgac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgaga gagcggaggt
      781 agcgcatccg gcggaagcgg aggagccatg tcctacgtga aggatgacat cttccgaatc
      841 tacattaaag agaaaaaaga gtgccggcgg gaccaccgcc caccgtgtgc tcaggaggcg
      901 ccccgcaaca tggtgcaccc caatgtgatc tgcgatggct gcaatgggcc tgtggtagga
      961 acccgctaca agtgcagcgt ctgcccagac tacgacttgt gtagcgtctg cgagggaaag
     1021 ggcttgcacc gggggcacac caagctcgca ttccccagcc ccttcgggca cctgtctgag
     1081 ggcttctcgc acagccgctg gctccggaag gtgaaacacg gacacttcgg gtggccagga
     1141 tgggaaatgg gtccaccagg aaactggagc ccacgtcctc ctcgtgcagg ggaggcccgc
     1201 cctggcccca cggcagaatc agcttctggt ccatcggagg atccgagtgt gaatttcctg
     1261 aagaacgttg gggagagtgt ggcagctgcc cttagccctc tgggcattga agttgatatc
     1321 gatgtggagc acggagggaa aagaagccgc ctgacccccg tctctccaga gagttccagc
     1381 acagaggaga agagcagctc acagccaagc agctgctgct ctgatcccag caagccgggt
     1441 gggaatgttg agggcgccac gcagtctctg gcggagcaga tgagaaagat cgccttggag
     1501 tccgaggggc gccctgagga acagatggag tcggataact gttcaggagg agatgatgac
     1561 tggacccatc tgtcttcaaa agaagtggac ccgtctacag gtgaactcca gtccctacag
     1621 atgccagaat ccgaagggcc aagctctctg gacccctccc aggagggacc cacagggctg
     1681 aaggaagctg ccttgtaccc acatctcccg ccagaggctg acccgcggct gattgagtcc
     1741 ctctcccaga tgctgtccat gggcttctct gatgaaggcg gctggctcac caggctcctg
     1801 cagaccaaga actatgacat cggagcggct ctggacacca tccagtattc aaagcatccc
     1861 ccgccgttgt gagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     1921 ataactgatc ataatcagcc at
//
