LOCUS       sequence-85991-e        2177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2177)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(21..23,24..26,27..737)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca
      481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      541 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtcc ggactcagat ctggcagcgg tggaggcagc gcatccggcg
      781 gaagcggaag cgtgcgtgag tgcatctcca tccacgttgg ccaggctggt gtccagattg
      841 gcaatgcctg ctgggagctc tactgcctgg aacacggcat ccagcccgat ggccagatgc
      901 caagtgacaa gaccattggg ggaggagatg actccttcaa caccttcttc agtgagacgg
      961 gcgctggcaa gcacgtgccc cgggctgtgt ttgtagactt ggaacccaca gtcattgatg
     1021 aagttcgcac tggcacctac cgccagctct tccaccctga gcagctcatc acaggcaagg
     1081 aagatgctgc caataactat gcccgagggc actacaccat tggcaaggag atcattgacc
     1141 ttgtgttgga ccgaattcgc aagctggctg accagtgcac cggtcttcag ggcttcttgg
     1201 ttttccacag ctttggtggg ggaactggtt ctgggttcac ctccctgctc atggaacgtc
     1261 tctcagttga ttatggcaag aagtccaagc tggagttctc catttaccca gcaccccagg
     1321 tttccacagc tgtagttgag ccctacaact ccatcctcac cacccacacc accctggagc
     1381 actctgattg tgccttcatg gtagacaatg aggccatcta tgacatctgt cgtagaaacc
     1441 tcgatatcga gcgcccaacc tacactaacc ttaaccgcct tattagccag attgtgtcct
     1501 ccatcactgc ttccctgaga tttgatggag ccctgaatgt tgacctgaca gaattccaga
     1561 ccaacctggt gccctacccc cgcatccact tccctctggc cacatatgcc cctgtcatct
     1621 ctgctgagaa agcctaccat gaacagcttt ctgtagcaga gatcaccaat gcttgctttg
     1681 agccagccaa ccagatggtg aaatgtgacc ctcgccatgg taaatacatg gcttgctgcc
     1741 tgttgtaccg tggtgacgtg gttcccaaag atgtcaatgc tgccattgcc accatcaaaa
     1801 ccaagcgcag catccagttt gtggattggt gccccactgg cttcaaggtt ggcatcaact
     1861 accagcctcc cactgtggtg cctggtggag acctggccaa ggtacagaga gctgtgtgca
     1921 tgctgagcaa caccacagcc attgctgagg cctgggctcg cctggaccac aagtttgacc
     1981 tgatgtatgc caagcgtgcc tttgttcact ggtacgtggg tgaggggatg gaggaaggcg
     2041 agttttcaga ggcccgtgaa gatatggctg cccttgagaa ggattatgag gaggttggtg
     2101 tggattctgt tgaaggagag ggtgaggaag aaggagagga atactaagga tccaccggat
     2161 ctagataact gatcata
//
