LOCUS       sequence-85992-e        1912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1912)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-VASP-N-10
FEATURES             Location/Qualifiers
     source          1..1912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1164..1173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1170..1172,1173..1175,1176..1889)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1215..1236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1476..1495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1823..1844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagcgatgc caccatgagc gagacggtca tctgttccag ccgggctact gtgatgcttt
       61 atgatgacag caacaagcga tggctccctg ctggcactgg tccccaggcc ttcagccgcg
      121 tccagatata ccacaacccc actgctaact ccttccgagt tgttggccgc aagatgcagc
      181 cggatcagca ggtggttatc aactgtgcca tcattcgggg tgtcaagtac aatcaggcca
      241 ctcccatctt ccatcagtgg cgagatgccc gccaggtctg gggcctcaac ttcggcagca
      301 aggaggacgc catacagttt gcaacaggca tggccaacgc cctagaggcc ttggaaggag
      361 gtgggcctcc cccagcccca gcaccccctg cctggtctgc ccagaatggt ccctccccag
      421 aggagctgga acaacagaaa aggcagccgg agcatatgga gcgccgggtc tccaatgcag
      481 gaggcccacc tgctccccca gctgggggcc ctcctccacc tccaggacct ccccctcctc
      541 caggtccccc cccaccccca ggtctgccct cctcaggggt atctggggca ggtcatggag
      601 caggggcagc cccaccccct gcacccccac tccctacaac acagggcccc aatagtgggg
      661 gttccggggc cccaggcctg gctgctgcca ttgctggagc caaactcagg aaagtgagca
      721 agcaggagga ggcctctggg gggcccctgg cccccaaagc tgagaacagt cgaagcactg
      781 gtggggggct tatggaagag atgaacgcca tgctggcccg gagaagaaaa gccacacagg
      841 ttggggagaa gccccccaaa gacgagtcag ccagtgagga gtcagaggcc cgactccctg
      901 cccagagtga acctgtgaga agaccctggg agaagaacag cacaaccttg ccaaggatga
      961 agtcgtcctc ttctgtgact acctccgagg cccacccctc gacgccctgc tccagtgatg
     1021 actccgactt ggagagggtg aagcaggagc ttctggaaga ggtgcggaag gagctacaga
     1081 aaatgaaaga ggaaatcatc gaagtctttg tccaggagct gaggaagcgg ggttctccta
     1141 gctccggagg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     1201 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1261 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1321 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt
     1381 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1441 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1501 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1561 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agcgacaacg
     1621 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     1681 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1741 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     1801 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1861 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca ta
//
