LOCUS       sequence-85999-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal chromosomal tagging with stable GFP under control of GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  TITLE     Pringle Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(83..524)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(89..112)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    407..524
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        658..1002
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 attcacaaga attgggacaa ctccagtgaa aagttcttct cctttactgt taattaaaga
       61 cattttgaga tccgggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt
      121 tttgttgata cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt
      181 attagttaaa gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca
      241 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
      301 atggttgtta atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt
      361 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
      421 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag
      481 tcttccttcg gagggctgtc acccgctcgg cggcttctaa tccgtacttc aatatagcaa
      541 tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg ctaagataat
      601 ggggctcttt acagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
      661 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
      721 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
      781 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
      841 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
      901 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcctt
      961 gctaggatac agttctcaca tcacatccga acataaacaa ccatgg
//
