LOCUS       sequence-86001-e        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chromosomal tagging with stable GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, 
            Pringle JR
  TITLE     Identification of yeast IQGAP (Iqg1p) as an 
            anaphase-promoting-complex substrate and its role in 
            actomyosin-ring-independent cytokinesis.
  JOURNAL   Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17.
  PUBMED    17942599
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-12"; volume: "18(12)"; pages: "5139-53. Epub 
            2007 Oct 17"
COMMENT     Sequence Label: pFA6a-GFP (F64L, S65T, V163A)-kanMX6
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      34..221
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(89..113)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        296..639
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(707..726)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actatacaaa tagggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
       61 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
      121 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
      181 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
      241 agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
      301 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
      361 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
      421 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
      481 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
      541 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
      601 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga aaagactcac
      661 gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
      721 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
      781 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
      841 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
      901 actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta
      961 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1021 cggttgcatt cgattcc
//
