LOCUS       sequence-86004-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chromosomal tagging with stable GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, 
            Pringle JR
  TITLE     Identification of yeast IQGAP (Iqg1p) as an 
            anaphase-promoting-complex substrate and its role in 
            actomyosin-ring-independent cytokinesis.
  JOURNAL   Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17.
  PUBMED    17942599
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-12"; volume: "18(12)"; pages: "5139-53. Epub 
            2007 Oct 17"
COMMENT     Sequence Label: pFA6a-GFP (F64L, S65T, V163A)-TRP1
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(115..133)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(116..133)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catctaattc acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactgttaat
       61 taacccgggg atccgtcgac ctgcagcgta cgaagcttca gctggcggcc gcgttctata
      121 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
      181 taacgacaat atgtccatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
//
