LOCUS       sequence-86007-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chromosomal tagging with stable GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, 
            Pringle JR
  TITLE     Identification of yeast IQGAP (Iqg1p) as an 
            anaphase-promoting-complex substrate and its role in 
            actomyosin-ring-independent cytokinesis.
  JOURNAL   Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17.
  PUBMED    17942599
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-12"; volume: "18(12)"; pages: "5139-53. Epub 
            2007 Oct 17"
COMMENT     Sequence Label: pFA6a-GFP (F64L, S65T, V163A)-His3MX6
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(105..123)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(106..123)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(204..223)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     323..345
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(383..401)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        469..573
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(792..811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactgttaat taacccgggg
       61 atccgtcgac ctgcagcgta cgaagcttca gctggcggcc gcgttctata gtgtcaccta
      121 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
      181 atgtccatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      241 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      301 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      361 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      421 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      661 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      721 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      781 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      841 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      901 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      961 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1021 ttgcacaaca tgggggatca tgtaactcgc cttgatc
//
