LOCUS       sequence-86010-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 
            UCS>mKate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ravikumar A, Arrieta A, Liu CC
  TITLE     An orthogonal DNA replication system in yeast.
  JOURNAL   Nat Chem Biol. 2014 Mar;10(3):175-7. doi: 10.1038/nchembio.1439. 
            Epub 2014 Feb 2.
  PUBMED    24487693
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1439"; journalName: "Nat Chem Biol"; date: 
            "2014-03"; volume: "10"; issue: "3"; pages: "175-7"
COMMENT     Sequence Label: pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             203..901
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MHVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRI
                     KAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVL
                     TATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMA
                     LKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVAR
                     YCDLPSKLGHR"
     primer_bind     complement(972..996)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcttc tagaagtact atcaaaatct gttattctat
       61 tgccttgagg atctttacac caatctgagt ctgaggacgc gtgatgacct atacatagga
      121 agatctatag aaacaaaaag attaataact ttcaaatatc agaaaaatgt agaaatatat
      181 gataagctca tagacatgta aaatgcatgt gagcgagctg attaaggaga acatgcacat
      241 gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga
      301 aggcaagccc tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct
      361 ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa
      421 ccacacccag ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga
      481 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
      541 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc
      601 tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagaccctgt accccgctga
      661 cggcggcctg gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat
      721 ctgcaacttg aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg
      781 cgtctactat gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctacgt
      841 cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacag
      901 aggatcctaa ctcgaggcga atttcttatg atttatgatt tttattatta aataagttat
      961 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     1021 attcttgagt aactctttcc tgtaggtca
//
