LOCUS       sequence-86011-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Left hand TALEN against Aedes aegypti KMO gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Aryan A, Anderson MA, Myles KM, Adelman ZN
  TITLE     TALEN-based gene disruption in the dengue vector Aedes aegypti.
  JOURNAL   PLoS One. 2013;8(3):e60082. doi: 10.1371/journal.pone.0060082. Epub 
            2013 Mar 21.
  PUBMED    23555893
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(3):e60082. doi"; pages: " 
            10.1371/journal.pone.0060082. Epub 2013 Mar 21"
COMMENT     Sequence Label: pSLfa PUb TAL-KMO-L
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(242..261)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    271..352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     296..315
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgacatgatt acgaattggc gcgcctaggc cggccgaatt caagatacat
      241 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      301 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
      361 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
      421 gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc cgcgtcgaca
      481 gatctgcagg ctcgaggagc tcgtctagag gatcgctcga gttatcagtc ggccgcgaag
      541 ttgatctcgc cgttgttgaa cttcctcctc acctcctcca gggtcagggt gccggccttg
      601 atcatctcgc cgccgatcag gagctcctcc acggacagca cggcgccgtt gcagttggtg
      661 atgtggttca gcctggtcag ctgggccttg tagttgccct tgaagtggcc ggacacgaac
      721 aggaacttga actcggtcac gctggagggg tacaccttcc accactcgtt ggggttgatg
      781 tgcttgttcc tggtctggtt ctcctccacg tacctctgca tttcgtcggc ctggccgatg
      841 ggcaggttgt a
//
